Descrizione del progetto
Decifrare la patogenesi dell’acidosi ruminale subacuta
L’acidosi ruminale subacuta (SARA, subacute ruminal acidosis) è un disturbo digestivo che colpisce le vacche da latte e influisce negativamente sulla salute degli animali e sulla redditività dell’industria in generale. Questa patologia si associa a un aumento nella concentrazione dei lipopolisaccaridi ruminali e i dati preliminari hanno messo in evidenza il fatto che questi composti chimici partecipano alla patogenesi della SARA attraverso un incremento dei batteri ruminali che producono lattato e digeriscono amido, nonché una sovraregolazione dei geni coinvolti nella biosintesi degli stessi lipopolisaccaridi. Gli obiettivi del progetto LIS, finanziato dall’UE, sono un approfondimento dettagliato del ruolo svolto dai lipopolisaccaridi nella patogenesi dell’acidosi ruminale subacuta e la fornitura di orientamenti per lo sviluppo di potenziali prodotti in grado di inattivarli all’interno del rumine.
Obiettivo
Subacute ruminal acidosis (SARA) is a well-recognized digestive disorder of high yielding dairy cows that has a negative impact on both animal health and herd profitability. It is associated with increased ruminal lipopolysaccharide (LPS), and LPS has been reported to participate in the pathogenesis of SARA. LPS from E. coli (LPS-E) has been used to study its effects on the SARA pathogenesis. Our preliminary data showed that LPS-E participates in the pathogenesis of SARA by directly increasing the starch-digesting and lactate-producing ruminal bacteria and the genes involved in the LPS biosynthesis process. However, we do not know if LPS-E would be representative of LPS from ruminal bacteria (LPS-R), and thus we do not know how LPS released from SARA cows participate in the pathogenesis of SARA. Therefore, the objectives of this proposal are to 1) isolate LPS from the rumen bacteria by inducing SARA nutritional model in batch culture (WP1, 2, 5, 6 & 7); 2) and compare the effects of LPS-R with the LPS-E on ruminal fermentation, bacterial community composition (BCC), and function by using 16S rRNA sequencing and RNA sequencing in batch culture to elucidate the roles of LPS on the pathogenesis of SARA (WP1, 3, 4, 5, 6 & 7). We hypothesis that 1) LPS from ruminal bacteria could be collected from culture media through inducing SARA nutritional model in batch culture; 2) LPS-R and LPS-E will have different effects on ruminal fermentation, BCC, and functions, but both of the two LPS sources would stimulate the bacteria and genes involved in starch digestion, lactic acid production, and LPS biosynthesis. We expect that our proposal could 1) fulfill the gap of knowledge of the role of LPS on the SARA pathogenesis; 2) provide the direction for industries to develop the products (e.g. clay) could inactive LPS in the rumen; 3) help European dairy industries save at least 994.4 million euros per year due to lower SARA prevalence.
Campo scientifico
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
- natural scienceschemical sciencesorganic chemistryorganic acids
- natural sciencesbiological sciencesmicrobiologybacteriology
- agricultural sciencesanimal and dairy sciencedairy
- natural sciencesbiological sciencesgeneticsRNA
- engineering and technologyindustrial biotechnologybioprocessing technologiesfermentation
Parole chiave
Programma(i)
Argomento(i)
Meccanismo di finanziamento
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinatore
20122 Milano
Italia