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Dysfunctional mitochondria as a neuronal pathogen

Descripción del proyecto

ARN mitocondrial como responsable de la enfermedad mitocondrial

Las mitocondrias son orgánulos eucariotas que generan la mayoría de la energía de las células. La disfunción mitocondrial afecta al metabolismo celular y conduce a la enfermedad mitocondrial (EM), que parece afectar especialmente al sistema nervioso, gran consumidor de energía. El equipo del proyecto MitoTROJAN, financiado con fondos europeos, trabajará con la hipótesis de que el ARN de doble cadena mitocondrial (mtdsRNA, por sus siglas en inglés) está relacionado con la neuropatología de la EM. Los investigadores aplicarán un enfoque multidisciplinario y, mediante un modelo murino de EM, estudiarán los mecanismos de respuesta inmunitaria asociados con el mtdsRNA. Los resultados ampliarán los conocimientos disponibles actualmente sobre la etiología de la EM e identificarán nuevas dianas terapéuticas que puedan aplicarse a casos de neurodegeneración.

Objetivo

The symbiosis between the mitochondrion and the ancestor of the eukaryotic cell allowed cellular complexity. Due to its archeobacterial origin, mitochondria has been posited as a potential source of molecules that can elicit cellular responses to pathogens. Among other key functions, mitochondria generates most of the energy required by cells. Alterations in components of the mitochondrial energy-generating machinery lead to primary mitochondrial disease (MD), a group of highly invalidating human conditions with no effective treatment. High-energy-requiring cells, such as neurons, are especially affected in MD. However, not all neuronal populations are equally affected and the molecular determinants of this susceptibility are currently unknown. Recently, Quintana´s lab uncovered that GABAergic neurons in the Globus Pallidus (GPe) of a mouse model of Leigh syndrome (LS) are particularly sensitive to MD leading to fatal encephalopathy, recapitulating the human pathology. In addition, unpublished results of the host group showed that this neuronal population develop a robust cellular antiviral-like response elicited by mitochondrial double-stranded RNA (mtdsRNA). Nevertheless, the contribution of mtdsRNA release in the MD progression has never been defined. Thus, the main goal of MitoTROJAN is to identify the effect of mtdsRNA-induced neuropathology in the context of mitochondrial dysfunction with the overarching goal of providing novel targets for the treatment of MD. By combining mouse genetics with cutting-edge molecular biology and multi-omics approach, I will carry out a multidisciplinary strategy to i) parse the molecular mechanisms of mtdsRNA-induced neuropathology and ii) characterise the antiviral response-induced protein shutdown in affected neurons. Overall results will provide new insight on an uncharacterized mechanism of mitochondria-mediated neurodegeneration and will help to identify novel therapeutic targets for MD and other neurodegenerative diseases.

Coordinador

UNIVERSITAT AUTONOMA DE BARCELONA
Aportación neta de la UEn
€ 172 932,48
Dirección
EDIF A CAMPUS DE LA UAB BELLATERRA CERDANYOLA V
08193 Cerdanyola Del Valles
España

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Región
Este Cataluña Barcelona
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 172 932,48