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Fucoxanthin Upregulation through Combined Omics and Metabolically Improving Control Segments

Project description

Optimisation of fucoxanthin production in diatom microalgae

Recent trends in health and disease prevention have led to an increase in the demand for naturally synthesised nutraceuticals, particularly the carotenoid fucoxanthin. Diatom microalgae belonging to the Phaeodactylum genus synthesise high amounts of fucoxanthin, making them one of the most promising candidates for sustainable production. Funded by the Marie Skłodowska-Curie Actions programme, the FUCOMICS project aims to develop an integrated omics platform to better understand fucoxanthin synthesis and identify regulatory elements/genes for enhancing this process in the Norwegian isolate Phaeodactylum tricornutum B58. Culture conditions will be optimised for maximum fucoxanthin synthesis using response surface methodology, and the identified regulators will be validated via advanced CRISPR-Cas9 technology.

Objective

The growing awareness towards health and disease prevention holds a great future globally for naturally derived nutraceuticals. This has led to a dramatic increase in demands of naturally synthesized carotenoids, particularly fucoxanthin.
This golden-brown carotenoid is used as anti-oxidant, anti-cancer, anti-diabetic, and anti-obesity component making it a valuable product for nutraceutical and food industries. Diatoms, particularly Phaeodactylum, naturally synthesize fucoxanthin in high amounts as compared to their counterparts (seaweeds), making them one of the most promising candidates for sustainable fucoxanthin production. However, to exploit these diatoms for commercial fucoxanthin production, highly productive strains capable of growing at low operational costs along with a detailed understanding of the fucoxanthin biosynthesis pathway to steer conditions towards most favourable for high yields are imperative. To this end, FUCOMICS will develop an integrated omics pipeline (transcriptomics and proteomics) to gain insight into fucoxanthin synthesis and identify key regulatory elements/genes for enhancing fucoxanthin synthesis in the local Norwegian isolate Phaeodactylum tricornutum B58. Further, by utilizing Response Surface Methodology culture conditions will be optimized for maximum fucoxanthin synthesis. The identified key regulatory elements/genes will be validated by generating engineered strains of P.
tricornutum B58 using the advanced CRISPR/Cas 9 technique. The results generated by FUCOMICS will not only be a starting step towards commercialization of microalgal fucoxanthin, but can also act as foundation for more difficult components, such as omega-3 fatty acids and biomass as whole. Furthermore, the fucoxanthin-extracted biomass, still rich in omega-3 fatty acids, can be utilized as feedstock for aquaculture, a major industry in Norway. As such, this project is in line with the EU strategy for the sustainable development of algae and aquaculture.

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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(opens in new window) H2020-MSCA-IF-2020

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Coordinator

UNIVERSITETET I BERGEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 214 158,72
Address
MUSEPLASSEN 1
5020 Bergen
Norway

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Region
Norge Vestlandet Vestland
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 214 158,72
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