MINFLUX super-resolution microscopy for improved stability and accessibility
The minimal photon fluxes (MINFLUX) concept enables 3D spatial fluorescence microscopy at molecular-scale resolution (1–3 nm) combined with millisecond temporal resolution in living cells. MINFLUX obtains information about dynamic functional molecular changes, yet current commercial systems are prohibitively expensive. Funded by the Marie Skłodowska-Curie Actions programme, the RobMin project will develop a MINFLUX system with simplified optical scanning, making it more affordable, easier to build and accessible to a broad range of researchers. RobMin will replace the 3D scanning with a combination of spatial light modulators and fast switching of multiple optical fibres, preserving imaging speed and resolution. The capabilities of the modified MINFLUX system will be tested on biological objects.