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Memory B cell dynamics in the gut mucosa

Project description

Memory B cell dynamics in the context of gastrointestinal infection

Foodborne salmonellosis, caused by the gram-negative bacteria Salmonella, affects millions of people every year, and there is no licensed vaccine available. Most human vaccines rely on the induction of long-lived memory lymphocytes. Memory B lymphocytes can rapidly differentiate into antibody-secreting cells and respond following exposure to pathogens. However, there is very limited knowledge of the dynamics of memory B cells in cases of gastrointestinal infection. Funded by the Marie Skłodowska-Curie Actions programme, the GUT-MBC project aims to unravel how memory B cells are generated and maintained in the gut mucosa and associated lymphoid tissues during Salmonella infection. To that end, researchers will study the developmental kinetics, spatial distribution and tissue diversity of memory B cells in the gastrointestinal tract.

Objective

Salmonellosis is one of the most common and widespread food-borne diseases worldwide. This disease, caused by the gram-negative bacteria Salmonella, affects millions of people every year, leading to more than one hundred thousand deaths around the world. Currently, there is no licensed vaccine against Salmonella to face out this health issue.
At present, most licensed human vaccines rely on the induction of long-lived memory lymphocytes. Memory B cells are a key part of this strategy, as they can rapidly differentiate into antibody-secreting cells and neutralize pathogens in case of future exposures. Despite their protective role, we have very limited knowledge on the dynamics of memory B cells in the context of gastrointestinal infection.
GUT-MBC aims to unravel the developmental kinetics, spatial distribution and tissue diversity of memory B cells in the gastrointestinal tract upon Salmonella infection. To achieve this goal, I will take advantage of a transgenic mouse line to track memory B cells in vivo, gastrointestinal infection models, multiparametric flow cytometry, cutting edge imaging techniques and single-cell RNA-seq. Understanding how memory B cells are generated and maintained in the gut mucosa and associated lymphoid tissues upon infection is critical for the future design of protective vaccines against Salmonella and other emerging enteric pathogens.

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2020

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Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 184 707,84
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 184 707,84
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