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Tools to access and analyze un-reduced mass spectrometry data to accelerate access to biotherapeutics

Project description

Innovative tools for structural analysis of biotherapeutics

Advances in biotherapeutics-based treatments for human diseases including COVID-19 are crucial. Mass spectrometry (MS) is a powerful technique for supporting the development of biotherapeutics-based drugs and clinical diagnosis. However, even versatile high-performance Orbitrap Fourier transform mass spectrometry (FTMS) may show limitations in the structural analysis of complex biotherapeutics, such as monoclonal antibodies and antibody-drug conjugates. The EU-funded A2MStools project identified the absence of hardware and software tools needed to acquire and analyse the unreduced time-domain data as the restricting factor in biotherapeutics structure analysis. The project will investigate the added value of an access to the existing but currently unavailable unreduced time-domain data for the Orbitrap FTMS platforms and allied software tools to handle these (big) data sets.

Objective

Biotherapeutics, such as monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs), constitute an increasingly important class of molecular drugs. Accelerating our society’s access to novel biotherapeutics-based therapies and diagnostic tests for existing and new clinical indications, including those caused by COVID-19, is critical. The exceptional specificity and selectivity of mass spectrometry (MS) renders this analytical technique a powerful tool in development of biotherapeutics-based drugs and clinical diagnostics. Nevertheless, even Orbitrap Fourier transform mass spectrometry (FTMS), which dominates the high-performance MS field, may demonstrate limited performance in structural analysis of the very complex mAbs and ADCs. Indeed, while the user-accessible Orbitrap data (processed mass spectra with reduced information) may be of a sufficient quality and information content for small molecule and isolated proteins analysis, it is not for the complex biotherapeutics. We identified the limiting factor that inhibits biotherapeutics structure analysis being the absence of hardware and software tools to acquire (access and provide to the end-users) and analyze the un-reduced data (e.g. time-domain signals or transients). With these tools in hands, the end-users would be able to increase performance and productivity of their biopharma workflows on the Orbitraps (e.g. leading to increased sensitivity and resolution, and thus leading to faster identification of more species and their deeper sequencing), as well as create novel workflows, not possible otherwise. The A2MStools (access & analysis MS tools) aims to address this limitation by: (i) providing access to the currently unavailable un-reduced data (transients) for the Orbitraps FTMS platforms with enabled BioPharma capabilities, and (ii) empowering analytical scientists with the data analysis tools capable of handling the datasets of unprocessed data in the most comprehensive and innovative ways.

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Topic(s)

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Funding Scheme

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CSA-LSP - Coordination and support action Lump sum

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Call for proposal

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(opens in new window) H2020-FETOPEN-2018-2020

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Coordinator

SPECTROSWISS SARL
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 100 000,00
Address
EPFL INNOVATION PARK BUILDING I
1015 Lausanne
Switzerland

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Schweiz/Suisse/Svizzera Région lémanique Vaud
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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