Objective
Chemical reactions govern cellular behavior, and are revealed by analysis of small molecules involved in intracellular metabolism. Individual cells in biological systems continuously adapt to improve survival and biological function, making them chemically and behaviorally heterogeneous. Unraveling this heterogeneity is essential to realize the correlation to disease state and health, but it is masked in bulk analyses of millions of cells.
I propose to develop a groundbreaking analytical approach for multi-omics of living individual cells to reveal variability in cellular behavior. This will be achieved by coupling a microfluidic device that enables controlled chemical exposure of a cell, to an efficient ionization probe for on-line time-resolved mass spectrometric measurements. By measuring the dynamics of each cell’s metabolome, lipidome, and secretome, novel insights into heterogeneity in intracellular activities will be gained. In addition, the level of heterogeneity will be uncovered through correlation with the cell’s transcriptome.
A special emphasis will be given to characterize individual β-cells that are key regulators of blood glucose by insulin secretion and whose dysfunction leads to type 2 diabetes. The behavior of individual β-cells is heterogeneous and ranges from complete failure to secrete insulin to compensating with increased secretion. I will use the single-cell multi-omics approach to test the hypothesis that intracellular metabolism is the key to β-cell dysfunction, and analyze healthy and diabetic β-cells upon chemical exposure to establish i) their metabolic heterogeneity and differences, ii) variations and temporal dynamics in their metabolic behavior and iii) metabolic roadblocks that correlate with β-cell dysfunction.
The single-cell multi-omics approach will open new horizons for understanding cellular heterogeneity, realizing cellular behavior that promotes health, and identifying treatment targets.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine endocrinology diabetes
- natural sciences biological sciences genetics RNA transcriptomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2021-STG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
751 05 Uppsala
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.