Description du projet
Dévoiler les interactions cellulaires au cours du développement
Les cellules de la crête neurale sont un groupe temporaire de cellules dans l’embryon en développement qui vont générer différents tissus. Les cellules de la crête neurale migrent le long de différentes voies où elles rencontrent des micro-environnements censés jouer un rôle clé dans la détermination du destin cellulaire. Financé par le Conseil européen de la recherche, le projet CELLMATES s’intéresse à la délimitation de ces interactions cellule-cellule au niveau transcriptionnel. Grâce à une nouvelle approche, les chercheurs obtiendront des informations transcriptomiques sur le micro-environnement et identifieront son rôle fonctionnel dans les décisions relatives au destin des cellules. Les résultats contribueront à une meilleure compréhension des interactions cellulaires dans l’espace et le temps.
Objectif
Multicellular life relies on coordinated physical interactions and communication between cells. In these interactions, cells exchange information that determines their fate, physiological state and behavior. The substantial transcriptional heterogeneity that is evident even among cells considered the same type, however, raises questions about exactly which temporal and spatial interactions regulate development and homeostasis. During development, for instance, multipotent neural crest cells migrate along different pathways in which they encounter microenvironments that are thought to instruct their migration and fate choices. To ultimately reveal how cell communication shapes cell identities and cellular decisions, we need to know the transcriptional state of a cell and that of its neighbors. Current technologies either sequence single cells at high transcriptional resolution or localize cells in their spatial context but without capturing their precise transcriptional configuration. To overcome these limitations, we will establish a novel approach that provides high-resolution transcriptomic information on cellular microenvironments. We will use this approach to uncover the interactions and communication codes that neural crest cells are exposed to along their developmental path. In addition, we will develop strategies to systematically identify cell-cell interactions likely to determine migration and cell fate decisions, and elucidate their functional role in the developing embryo. Our overall goal is to uncover how the diversity of cell interactions orchestrates the development of a complex cell lineage. This approach and the insights obtained here will provide a foundation for studying how cells interact in other systems such as human tissues, and how these interactions are altered in disease.
Champ scientifique
Mots‑clés
Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Thème(s)
Régime de financement
HORIZON-ERC - HORIZON ERC GrantsInstitution d’accueil
10117 Berlin
Allemagne