Descripción del proyecto
Información sobre las proteínas de unión al ARN bacteriano
Las proteínas de unión al ácido ribonucleico (ARN) (RNP o RBP por sus siglas en inglés) se están posicionando como reguladores básicos de los procesos postranscripcionales que afectan funciones biológicas importantes. Las RNP contienen dominios de unión a ARN o regiones altamente desordenadas responsables de interactuar con moléculas de ARN, como los transcritos. En el proyecto bacRBP, financiado con fondos europeos, se examinan RNP no convencionales poco estudiadas de bacterias que carecen de dominios de unión a ARN. Sus investigadores emplearán un método innovador desarrollado hace poco para capturar y estudiar estas RNP. El objetivo es identificar las RNP implicadas en las respuestas al estrés y en la infección. Los resultados del proyecto proporcionarán conocimientos fundamentales sobre el control celular y posibilitarán el diseño de nuevos tratamientos antimicrobianos.
Objetivo
All organisms use diverse modes of cellular control as they cope with changing environments. Central to these processes are RNA-binding proteins (RBPs) that impact the stability, translation, or localization of bound RNAs. While RBPs typically have distinct RNA-binding domains, a growing number of proteins that lack these domains are found to interact with RNA as well. In prokaryotes, such unconventional RBPs remain largely unexplored, in part because methods for global RNA interactome capture (RIC) in bacteria are missing. My group recently made a breakthrough in developing a novel RIC approach for bacteria that relies on primary transcript capture (CoCAP). Our pilot study successfully captured known RBPs but also uncovered numerous new RBP candidates, including metabolic or cell division proteins. We also identified a pair of widespread KH-domain proteins (KhpA/B) with links to the RNA degradosome and cell division. This points towards a wealth of unexplored RBPs involved in cellular control in bacteria.
My ERC CoG proposal aims to explore the identity and functional diversity of novel RBPs in bacteria. My overarching hypothesis is that a vast, unexplored universe of unconventional RBPs exists in bacteria that play crucial roles in cellular physiology. I will tackle this through three objectives leveraging two model bacteria (Salmonella and Campylobacter) with different sets of canonical RBPs. I propose to:
1) Elucidate bacterial primary RBPomes during stress- and infection-relevant conditions.
2) Identify mechanisms and cellular functions of two widely conserved KH-domain RBPs.
3) Determine how cell division RBPs influence and are influenced by bound RNAs.
Our proposed work will provide a broadly applicable method for primary RBPome capture and vastly expand the set of bacterial RBPs. Their characterization in turn will reveal new layers of cellular control and establish new targets for industrial strain engineering and antimicrobial treatments.
Ámbito científico
Palabras clave
Programa(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régimen de financiación
HORIZON-ERC - HORIZON ERC GrantsInstitución de acogida
97070 Wuerzburg
Alemania