Descripción del proyecto
Modelo innovador de organoide cerebral no animal para la investigación de la neurodegeneración
El envejecimiento de la población europea conlleva un aumento creciente de la prevalencia de enfermedades neurodegenerativas (END) asociadas a la demencia y la pérdida de funciones motoras en el caso de la enfermedad de Parkinson. El objetivo del proyecto OpenMIND, financiado con fondos europeos, es introducir un concepto innovador en la modelización de enfermedades denominado «conectoide». En este sentido, se creará un modelo de organoide cerebral multirregional optoelectrónico humano «in vitro» de conectoides formados por organoides cerebrales conectados a través de haces de hidrogel para la señalización funcional axonal. Cada organoide contendrá neuronas específicas controladas por luz y electrodos penetrantes, así como guías de onda, para supervisar la señalización de neurotransmisores dentro y entre los organoides. Este modelo revolucionario permitirá visualizar y evaluar al instante las respuestas de regiones cerebrales concretas a un determinado tratamiento.
Objetivo
A major challenge facing Europe is its ageing population and associated increase in diagnosed cases of neurodegenerative diseases (NDD). Parkinson’s disease (PD) is associated with tremor and loss of motor functions due to progressive degeneration of dopaminergic neurons in the brain. This can lead to memory loss and dementia, which is associated with short- and long-term injuries and disabilities with emotional, financial, and social burdens for patients, families, and society. The exact causes and mechanisms underlying PD are still unknown and existing treatments focus on alleviating symptoms and increasing quality of life, but do not halt or reverse disease progression. Although animal models give unique possibilities to study physiological and behavioural mechanisms, drug development fails due to lack of translation to humans. Alternative non-animal NDD models is needed both in terms of better translation, but also to replace expensive and problematic animal experiments.
We will move disease modelling to a new level and replace animal models, by creating a new concept we call connectoids. We will develop an ex-vivo-type in vitro human opto-electronic multi-regional brain-organoid disease model in which connectoids are formed by precise spatial arrangement of brain organoids connected via hydrogel tracts that promote axonal pathfinding, functional connection, and signalling. By developing 1) light controllable sub-type specific neurons within regionalized brain organoids, and 2) electrodes and waveguides that can penetrate the organoids able to monitor neurotransmitter signalling inside and between the organoids, we will for the first time be able to sense how a particular brain region responds to a certain therapy and watch in real time how signals are transmitted to other brain regions. Our model will not only have health benefit, but will relieve a heavy economic burden on society, and open up for new possibilities for technological and economic development.
Ámbito científico
Palabras clave
Programa(s)
Convocatoria de propuestas
HORIZON-EIC-2021-PATHFINDEROPEN-01
Consulte otros proyectos de esta convocatoriaRégimen de financiación
HORIZON-EIC - HORIZON EIC GrantsCoordinador
2800 Kongens Lyngby
Dinamarca