Yeast cell factory for mRNA bioproduction

Livrables

Contains different materials to be used by the project partners for communication Activities incl key design elements logo colour scheme fonts and materials such as eg posters leaflets fact sheets or infographics

Project website and communication concept

Relevant stakeholders and potential user groups policy makers industry partners will be identified and addressed and the website will be presented

Publications

Molecular Therapy - Nucleic Acids

Auteurs: Andreja Krušič; Nina Mencin; Marta Leban; Evelin Nett; Mario Perković; Ugur Sahin; Polona Megušar; Aleš Štrancar; Rok Sekirnik
Publié dans: Molecular Therapy - Nucleic Acids, Numéro New York NY: Nature Pub. Group, 2025, ISSN 2162-2531
Éditeur: Nature Publishing Group
DOI: 10.1016/J.OMTN.2025.102491

Development and Scale-Up of Oligo-Dt Monolithic Chromatographic Column for Mrna Capture Through Understanding of Base-Pairing Interactions

Auteurs: Mencin, Nina; Štepec, Dona; Margon, Alja; Vidič, Jana; Dolenc, Darko; Simčič, Tina; Rotar, Sara; Sekirnik, Rok; Štrancar, Aleš; Černigoj, Urh
Publié dans: SSRN Electronic Journal, Numéro 304, 2022, ISSN 1873-3794
Éditeur: Elsevier
DOI: 10.1016/J.SEPPUR.2022.122320

Nasal Delivery of mRNA Using Thermoresponsive Gellable Aqueous Biphasic Systems

Auteurs: Bojan Kopilovic, Nabila Laroui, João A.P. Coutinho, Chantal Pichon, Mara G. Freire
Publié dans: Small, 2025, ISSN 1613-6810
Éditeur: Wiley
DOI: 10.1002/SMLL.202409527

Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.

Auteurs: Almeida C, Pedro AQ, Tavares APM, Neves MC, Freire MG
Publié dans: Frontiers in Bioengineering and Biotechnology, Numéro Volume 11, 2023, ISSN 2296-4185
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fbioe.2023.1037436

Maltodextrin-Modified Lipoplexes for Enhanced Mucosal Penetration and Efficient mRNA Delivery

Auteurs: Kopilovic, Bojan; Laroui, Nabila; Berchel, Mathieu; Jaffrès, Paul-Alain; Midoux, Patrick; Freire, Mara; Pichon, Chantal
Publié dans: Materials Today Bio, Numéro 33, 2025, ISSN 2590-0064
Éditeur: Elsevier
DOI: 10.1016/J.MTBIO.2025.101975

Corrigendum: Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation

Auteurs: Catarina Almeida; Augusto Q. Pedro; Ana P. M. Tavares; Márcia C. Neves; Mara G. Freire
Publié dans: Frontiers in Bioengineering and Biotechnology, Vol 11 (2023), 2023, ISSN 2296-4185
Éditeur: Frontiers in bioengineering and biotechnology
DOI: 10.3389/fbioe.2023.1213921

Scalable multimodal weak anion exchange chromatographic purification for stable mRNA drug substance

Auteurs: Polona Megušar, Rok Miklavčič, Matevž Korenč, Jure Ličen, Tina Vodopivec, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
Publié dans: Electrophoresis, Numéro 44, 2023, ISSN 1522-2683
Éditeur: Wiley
DOI: 10.1002/elps.202300106

High Recovery Chromatographic Purification of mRNA at Room Temperature and Neutral pH

Auteurs: Rok Miklavčič, Polona Megušar, Špela Meta Kodermac, Blaž Bakalar, Darko Dolenc, Rok Sekirnik, Aleš Štrancar, Urh Černigoj
Publié dans: International Journal of Molecular Sciences, Numéro 24, 2023, ISSN 1422-0067
Éditeur: MDPI AG
DOI: 10.3390/ijms241814267

A comparative analysis of mRNA enrichment strategies and guidance for improving their efficiency

Auteurs: Angelika Andrzejewska-Romanowska, Ewa Tykwińska, Paweł Śledziński, Katarzyna Pachulska-Wieczorek
Publié dans: Scientific Reports, Numéro 15, 2025, ISSN 2045-2322
Éditeur: Springer Science and Business Media LLC
DOI: 10.1038/S41598-025-02082-Z

Separation and Purification Technology

Auteurs: Nina Mencin, Dona Štepec, Alja Margon, Jana Vidič, Darko Dolenc, Tina Simčič, Sara Rotar, Rok Sekirnik, Aleš Štrancar, Urh Černigoj
Publié dans: Separation and Purification Technology, Numéro 304, 2023, ISSN 1383-5866
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2022.122320

Using thermoreversible aqueous biphasic systems and ionic liquids to improve the manufacturing of mRNA nanomedicines

Auteurs: Sousa, Maria I.; Silva, Luís C. V.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.
Publié dans: 2024
Éditeur: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Purification of RNA from recombinant lysates using biocompatible amino-acid-based ionic liquids

Auteurs: Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.
Publié dans: 2024
Éditeur: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Effective separation of nucleic acids from complex bacterial lysates using three-phase partitioning systems based on biocompatible ionic liquids

Auteurs: Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.
Publié dans: 2025
Éditeur: CICECO

Aqueous biphasic systems composed by biocompatible amino acid-based ionic liquids as integrated preservation-purification platforms for RNA

Auteurs: Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.
Publié dans: 2024
Éditeur: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Development of an integrated messenger RNA manufacturing process using thermoreversible aqueous biphasic systems

Auteurs: Sousa, Maria I.; Silva, Luís; Freire, Mara G.; Tavares, Ana P. M.; Silva, Francisca A. e; Pedro, Augusto Q.
Publié dans: 2024
Éditeur: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

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