Yeast cell factory for mRNA bioproduction

Deliverables

Contains different materials to be used by the project partners for communication Activities incl key design elements logo colour scheme fonts and materials such as eg posters leaflets fact sheets or infographics

Project website and communication concept

Relevant stakeholders and potential user groups policy makers industry partners will be identified and addressed and the website will be presented

Publications

Molecular Therapy - Nucleic Acids

Author(s): Andreja Krušič; Nina Mencin; Marta Leban; Evelin Nett; Mario Perković; Ugur Sahin; Polona Megušar; Aleš Štrancar; Rok Sekirnik
Published in: Molecular Therapy - Nucleic Acids, Issue New York NY: Nature Pub. Group, 2025, ISSN 2162-2531
Publisher: Nature Publishing Group
DOI: 10.1016/J.OMTN.2025.102491

Development and Scale-Up of Oligo-Dt Monolithic Chromatographic Column for Mrna Capture Through Understanding of Base-Pairing Interactions

Author(s): Mencin, Nina; Štepec, Dona; Margon, Alja; Vidič, Jana; Dolenc, Darko; Simčič, Tina; Rotar, Sara; Sekirnik, Rok; Štrancar, Aleš; Černigoj, Urh
Published in: SSRN Electronic Journal, Issue 304, 2022, ISSN 1873-3794
Publisher: Elsevier
DOI: 10.1016/J.SEPPUR.2022.122320

Nasal Delivery of mRNA Using Thermoresponsive Gellable Aqueous Biphasic Systems

Author(s): Bojan Kopilovic, Nabila Laroui, João A.P. Coutinho, Chantal Pichon, Mara G. Freire
Published in: Small, 2025, ISSN 1613-6810
Publisher: Wiley
DOI: 10.1002/SMLL.202409527

Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.

Author(s): Almeida C, Pedro AQ, Tavares APM, Neves MC, Freire MG
Published in: Frontiers in Bioengineering and Biotechnology, Issue Volume 11, 2023, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2023.1037436

Maltodextrin-Modified Lipoplexes for Enhanced Mucosal Penetration and Efficient mRNA Delivery

Author(s): Kopilovic, Bojan; Laroui, Nabila; Berchel, Mathieu; Jaffrès, Paul-Alain; Midoux, Patrick; Freire, Mara; Pichon, Chantal
Published in: Materials Today Bio, Issue 33, 2025, ISSN 2590-0064
Publisher: Elsevier
DOI: 10.1016/J.MTBIO.2025.101975

Corrigendum: Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation

Author(s): Catarina Almeida; Augusto Q. Pedro; Ana P. M. Tavares; Márcia C. Neves; Mara G. Freire
Published in: Frontiers in Bioengineering and Biotechnology, Vol 11 (2023), 2023, ISSN 2296-4185
Publisher: Frontiers in bioengineering and biotechnology
DOI: 10.3389/fbioe.2023.1213921

Scalable multimodal weak anion exchange chromatographic purification for stable mRNA drug substance

Author(s): Polona Megušar, Rok Miklavčič, Matevž Korenč, Jure Ličen, Tina Vodopivec, Urh Černigoj, Aleš Štrancar, Rok Sekirnik
Published in: Electrophoresis, Issue 44, 2023, ISSN 1522-2683
Publisher: Wiley
DOI: 10.1002/elps.202300106

High Recovery Chromatographic Purification of mRNA at Room Temperature and Neutral pH

Author(s): Rok Miklavčič, Polona Megušar, Špela Meta Kodermac, Blaž Bakalar, Darko Dolenc, Rok Sekirnik, Aleš Štrancar, Urh Černigoj
Published in: International Journal of Molecular Sciences, Issue 24, 2023, ISSN 1422-0067
Publisher: MDPI AG
DOI: 10.3390/ijms241814267

A comparative analysis of mRNA enrichment strategies and guidance for improving their efficiency

Author(s): Angelika Andrzejewska-Romanowska, Ewa Tykwińska, Paweł Śledziński, Katarzyna Pachulska-Wieczorek
Published in: Scientific Reports, Issue 15, 2025, ISSN 2045-2322
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/S41598-025-02082-Z

Separation and Purification Technology

Author(s): Nina Mencin, Dona Štepec, Alja Margon, Jana Vidič, Darko Dolenc, Tina Simčič, Sara Rotar, Rok Sekirnik, Aleš Štrancar, Urh Černigoj
Published in: Separation and Purification Technology, Issue 304, 2023, ISSN 1383-5866
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2022.122320

Using thermoreversible aqueous biphasic systems and ionic liquids to improve the manufacturing of mRNA nanomedicines

Author(s): Sousa, Maria I.; Silva, Luís C. V.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.
Published in: 2024
Publisher: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Purification of RNA from recombinant lysates using biocompatible amino-acid-based ionic liquids

Author(s): Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.
Published in: 2024
Publisher: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Effective separation of nucleic acids from complex bacterial lysates using three-phase partitioning systems based on biocompatible ionic liquids

Author(s): Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.
Published in: 2025
Publisher: CICECO

Aqueous biphasic systems composed by biocompatible amino acid-based ionic liquids as integrated preservation-purification platforms for RNA

Author(s): Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.
Published in: 2024
Publisher: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Development of an integrated messenger RNA manufacturing process using thermoreversible aqueous biphasic systems

Author(s): Sousa, Maria I.; Silva, Luís; Freire, Mara G.; Tavares, Ana P. M.; Silva, Francisca A. e; Pedro, Augusto Q.
Published in: 2024
Publisher: CICECO - Centro de Investigação em Materiais Cerâmicos e Compósitos

Deliverables

Publications

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