Description du projet
Imagerie d’un virus unique
La lutte contre les infections virales par de nouveaux traitements nécessite la caractérisation des structures virales et l’identification de cibles thérapeutiques. Cependant, l’imagerie des virus ne peut être réalisée que dans des infrastructures de recherche spécialisées et exige des équipements coûteux. Pour remédier à cette contrainte, le projet NanoXCAN, financé par l’UE, mettra au point un nouveau microscope de table qui utilise les rayons X pour visualiser les virus. Le microscope sera capable d’effectuer de l’imagerie biomédicale à l’échelle nanométrique et sera mis à la disposition de la communauté scientifique au sens large. En dehors des études structurelles, cette technique peut être employée pour étudier les mécanismes impliqués dans l’infection virale ainsi que le mode d’action de nouveaux antiviraux.
Objectif
Coronavirus disease (COVID-19) is an infectious disease that emerged in late 2019. By March 2020, the outbreak was declared a devastating pandemic and clearly illustrated the threat that viruses pose to our society. The characterization of viral structures and the identification of key proteins involved in each step of the cycle of infection are crucial to developing treatments. Yet imaging single viruses can only be performed in a few specialized centers in Europe, while every hospital could benefit from it. NanoXCAN proposes to develop a tabletop virus imaging X-ray microscope, with foreseeable impact as revolutionary as the invention of super-resolved fluorescence microscopy, paving the way towards determination of structure and dynamics of matter to a large community. For this purpose, we will develop an original digital laser that delivers, on a daily operation, subwavelength focusing, reaching relativistic intensities at MHz repetition rates. This will be used to create a nano-source of hard X-rays from the Kalpha plasma emission of metallic nano-targets at an average power comparable to that of a synchrotron beamline. We will capitalize on this high brilliance, high average power hard X-ray source to perform lensless nanoscale biomedical imaging based on recent findings in incoherent imaging and machine learning. All these ingredients will create a unique nanoscopy platform that our consortium will illustrate by imaging a single virus. In the future, our X-ray IDI microscope could help to study mechanisms involved in viral infection and antiviral design. X-rays have the advantage of performing in-situ non-destructive and non-invasive imaging over competing techniques. NanoXCAN will create a transformative positive effect on our economy and society by proposing this new technology for single virus imaging.
Champ scientifique
- natural sciencesbiological sciencesmicrobiologyvirology
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- natural sciencesphysical sciencesopticsmicroscopy
- natural sciencescomputer and information sciencesartificial intelligencemachine learning
- natural sciencesphysical sciencesopticslaser physics
Programme(s)
Régime de financement
HORIZON-EIC - HORIZON EIC GrantsCoordinateur
1000-043 Lisboa
Portugal