Descrizione del progetto
Imaging di virus singoli
Per affrontare le infezioni virali ricorrendo a nuovi trattamenti è necessario caratterizzare le strutture virali e identificare i bersagli terapeutici. Tuttavia, l’imaging dei virus è un’attività eseguibile esclusivamente all’interno di infrastrutture di ricerca specializzate e richiede attrezzature costose. Per ovviare a questa limitazione, il progetto NanoXCAN, finanziato dall’UE, svilupperà un nuovo microscopio da tavolo che utilizza i raggi X per riprodurre una scansione dei virus. Il microscopio sarà in grado di eseguire imaging biomedico su scala nanometrica e sarà accessibile alla comunità scientifica in generale. Oltre agli studi strutturali, può essere impiegato per analizzare i meccanismi implicati nell’infezione virale nonché la modalità d’azione di nuovi antivirali.
Obiettivo
Coronavirus disease (COVID-19) is an infectious disease that emerged in late 2019. By March 2020, the outbreak was declared a devastating pandemic and clearly illustrated the threat that viruses pose to our society. The characterization of viral structures and the identification of key proteins involved in each step of the cycle of infection are crucial to developing treatments. Yet imaging single viruses can only be performed in a few specialized centers in Europe, while every hospital could benefit from it. NanoXCAN proposes to develop a tabletop virus imaging X-ray microscope, with foreseeable impact as revolutionary as the invention of super-resolved fluorescence microscopy, paving the way towards determination of structure and dynamics of matter to a large community. For this purpose, we will develop an original digital laser that delivers, on a daily operation, subwavelength focusing, reaching relativistic intensities at MHz repetition rates. This will be used to create a nano-source of hard X-rays from the Kalpha plasma emission of metallic nano-targets at an average power comparable to that of a synchrotron beamline. We will capitalize on this high brilliance, high average power hard X-ray source to perform lensless nanoscale biomedical imaging based on recent findings in incoherent imaging and machine learning. All these ingredients will create a unique nanoscopy platform that our consortium will illustrate by imaging a single virus. In the future, our X-ray IDI microscope could help to study mechanisms involved in viral infection and antiviral design. X-rays have the advantage of performing in-situ non-destructive and non-invasive imaging over competing techniques. NanoXCAN will create a transformative positive effect on our economy and society by proposing this new technology for single virus imaging.
Campo scientifico
- natural sciencesbiological sciencesmicrobiologyvirology
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- natural sciencesphysical sciencesopticsmicroscopy
- natural sciencescomputer and information sciencesartificial intelligencemachine learning
- natural sciencesphysical sciencesopticslaser physics
Parole chiave
Programma(i)
Invito a presentare proposte
HORIZON-EIC-2021-PATHFINDEROPEN-01
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