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Design, Build and Test of novel bacteria-delivered proteins for cancer therapy

Descripción del proyecto

Las bacterias administran tratamientos antineoplásicos

Ciertas cepas bacterianas tienen una capacidad inherente para atacar tumores y, en principio, pueden servir como estrategia antineoplásica alternativa. Sin embargo, la modificación estas bacterias como vehículos de administración de tratamientos antineoplásicos se ve limitada por la incapacidad de las sustancias terapéuticas de penetrar en las células cancerosas. El equipo del proyecto BacStar, financiado por las Acciones Marie Skłodowska-Curie, ha encontrado una solución. Los investigadores proponen diseñar y producir proteínas capaces de transportarse desde la célula bacteriana hasta la célula cancerosa. A través de una plataforma de diseño computacional de proteínas, identificarán proteínas con capacidad de transporte y actividad antineoplásica para su posterior ensayo experimental.

Objetivo

Conventional cancer therapies have many limitations that could potentially be overcome using engineered tumour targeting bacteria as cancer therapeutics. These bacteria have been shown to selectively grow in tumours where they can produce and deliver therapeutic biomolecules (proteins) extracellularly in the tumour microenvironment. However, the delivery of these biomolecules into the cytoplasm of targeted tumour cells remains a challenge, limiting the clinical translation of these therapies.

BacStar aims to address delivery challenges by designing multi-part, multifunctional proteins that transport themselves from the interior of the bacterium into the cytoplasm of cancer cells where they can exert therapeutic action. These proteins will integrate protein motifs for transport (using novel cell penetrating peptides) and anticancer activity, which will be assembled using recently developed computational protein design workflows. A modular design-build-test synthetic biology approach will facilitate measurability and experimental throughput. Novel proteins will be tested in vitro and in vivo with high-throughput methods available at host labs. This interdisciplinary approach is enabled by leveraging cutting-edge expertise and technologies in computational protein design from the Bhardwaj and Baker Lab at the Institute for Protein Design, UW (USA) and in bacterial cancer therapy available at the Tangney Lab, UCC (Ireland). An international intersectoral placement at Prokarium (UK) will support the clinical translation of outputs from this project and build my expertise in translational research, which will inform the commercialisation route of this and future projects.

My long-term career aim is to develop bacteria-delivered protein therapeutics. BacStar is a significant career opportunity to transition to independence with the active support of world leaders in their fields and gaining industry R&D experience which is key for the advance of these biotherapeutics.

Ámbito científico

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural.

Coordinador

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK
Aportación neta de la UEn
€ 328 494,96
Dirección
WESTERN ROAD
T12 YN60 Cork
Irlanda

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Región
Ireland Southern South-West
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
Sin datos

Socios (2)