Project description DEENESFRITPL Understanding wastewater as a reservoir for antibiotic resistance City sewers harbour complex microbial communities containing diverse antibiotic-resistant bacteria coming from human excreta. The MSCA-funded DEAR-Waste project seeks to understand the dynamics of antibiotic-resistant bacterial populations in city sewers by creating wastewater model systems (microcosms) that emulate the complexity of the sewage environment. Using interdisciplinary knowledge and analytical tools such as high-throughput sequencing and statistical modeling, it will emulate species performance under different chemical conditions and species interactions. These lab models will be compared with real-world measurements from city sewers. Coupled with the results of an evolutionary experiment using similar microcosms, the DEAR-Waste project will determine the effect of environmental complexity and interspecies interactions on the evolution of antibiotic resistance in urban wastewater. Show the project objective Hide the project objective Objective Environmental antibiotic resistance is a major threat to human and veterinary health and a key issue addressed by the European One Health Action Plan Against Antimicrobial Resistance. City sewers shelter rich and diverse bacterial communities that are continuously exposed to antibiotic residues from human excreta, thus becoming a reservoir of resistance. Predicting the risk of antibiotic resistance evolution in city sewers requires a comprehensive understanding of the dynamics and evolution of wastewater bacterial communities faced to such exposition. However, sewers are complex environments and contain multiple abiotic factors, which may act in non-additive ways. In addition, interactions between species within communities affect growth and consequently competition, through both density- and frequency-dependent processes. By changing the competitive ability of variants, such as antibiotic resistant phenotypes, interspecies interactions also change evolutionary processes.DEAR-Waste aims at understanding the dynamics of communities and antibiotic resistance evolution in city sewers, together with establishing wastewater as a model system for fundamental studies on community dynamics and evolution. DEAR-Waste adopts an interdisciplinary approach, combining high-throughput sequencing technologies, environmental chemistry, microbial ecology and evolution, time-series statistical analysis, and formal modelling. Data from synthetic mesocosms will parametrize the Hutchinsonian niche for population in communities: a tolerance curve mapping species performance onto a complex environmental space defined by the multidimensional abiotic environment and species interactions. Predictions from these curves will be tested against field measurements of microbial and chemical dynamics sampled in city sewers. An evolutionary experiment in similar mesocosms will finally quantify how environmental complexity and interspecies interactions modulate the evolution of antibiotic resistance. Fields of science engineering and technologyenvironmental engineeringwater treatment processeswastewater treatment processesnatural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesevolutionary biologymedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibioticsmedical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance Programme(s) HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme Topic(s) HORIZON-MSCA-2021-PF-01-01 - MSCA Postdoctoral Fellowships 2021 Call for proposal HORIZON-MSCA-2021-PF-01 See other projects for this call Funding Scheme HORIZON-AG-UN - HORIZON Unit Grant Coordinator FUNDACIO INSTITUT CATALA DE RECERCA DE L'AIGUA Net EU contribution € 181 152,96 Address Calle emili grahit edifici h20 101 17003 Girona Spain See on map Region Este Cataluña Girona Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window EU contribution No data
