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NeuroProtective role of HRI following cerebral ischemia

Project description

A targeted therapy for stroke

Stroke occurs when there is a block in the blood supply to a part of the brain. It affects more than one million people every year, and emergency treatments usually involve medication to resolve the blood clots that caused the stroke. Stroke care then on focuses on recovering as much of movement and sensation as possible through rehabilitation. Funded by the Marie Skłodowska-Curie Actions programme, the NeuProHRI project aims to develop a novel therapy for stroke that targets the heme regulated inhibitory (HRI) kinase cellular cascade. Researchers will employ different models of stroke to investigate the pathways that are activated by HRI kinase and test the outcome of compounds on the phenotype of stroke.

Objective

Nowadays, cerebrovascular diseases are devastating conditions representing a large cause of mortality worldwide. It is estimated that 1.1 million people suffer a stroke every year in Europe. Due to the lack of a reliable treatment for stroke, it is important to keep looking for new treatments that stop the progression of the insult by providing neuroprotection, and even promote neuronal recovery for those neurons already affected by the condition. The goal of NeuProHRI is to study the potential of targeting the HRI kinase cellular cascade as a new therapeutic aim for stroke. To develop this, I will use a combination of cutting-edge molecular techniques to define the cellular pathways underlying the activation of the HRI kinase, and their effect on promoting neuroprotection in in vitro and in vivo models of stroke. First, I will decipher the molecular cascade following the activation of HRI in primary cultured neurons by pharmacological and genetic approaches. Then, in vitro models of stroke will be used to measure the impact of these treatments in cells with stroke-like phenotype. Finally, mouse models of stroke will allow me to tackle the impact of pharmaceutical and genetic treatments on in vivo brain tissue. Moreover, Ribo-seq analysis will be performed to study accurately protein synthesis and its relation to neuron-intrinsic pathways involved in stroke. There is a clear benefit of the mobility for both the applicant and the host, ensuring high quality results and dissemination. In this regard, the host will ensure the acquisition of new technical, management, tutorial and transferable skills. The applicant will also benefit from a multidisciplinary environment enhancing international collaboration that will surely contribute to diversify his career. Finally, via a specialized Career Development Plan, the host will provide the ideal training and validation environment, through which the applicant will reach unprecedented levels of professional maturity.

Coordinator

FUNDACION PUBLICA GALEGA INSTITUTO DE INVESTIGACION SANITARIA DE SANTIAGO DE COMPOSTELA
Net EU contribution
€ 165 312,96
Address
TRAVESA DA CHOUPANA
15706 Santiago De Compostela
Spain

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Region
Noroeste Galicia A Coruña
Activity type
Research Organisations
Links
Total cost
No data

Partners (1)