CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.
Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .
Resultado final
The overall methodology includes (1) the re-analysis of RADseq data previously obtained by the host research group to quantify the connectivity among environments and neutral genome-wide levels of genetic diversity, (2) the sequencing of samples of adult fish from three different environments (Galicia and Norway for both; Celtic Sea for ballan wrasse, L. bergylta, and Balearic Sea for the hake, M. merluccius) and larvae from one of these environments (Galicia) to survey genomic functional Single Nucleotide Polymorphism (SNPs) by using HTS technologies to reveal the adaptive potential to environmental conditions, and (3) performing different genomic and statistical analyses to produce quantifiable estimates of the adaptive potential (i.e. the resilience index) and the effect of harvesting on it, and then the likelihood of adaptation to changes in the environment. RADseq data will be analysed using R packages such as ipyrad, Bayescan, Arlequin version 3.5.2.2, DNAsp. Statistics such as Fst-index, number of migrants, and genetic distances will be used to estimate the amount of gene flow among populations. DNA-seq reads obtained from sequencing of functional genome regions, will be managed and analyzed using Genome Analysis Toolkit framework (GATK). We plan to study a minimum of 10 groups of functionally related genes (i.e. grouped by gene-pathways or gene ontology) including ~ 500-1000 coding genes (~ 2.5 -5% of coding genes in the genome). Sequencing will cover the whole coding length of genes and 1kb of sequences upstream of the transcription start site in order to get putative regulatory sequences. In total, we plan to produce sequences of functional genomic regions for ~150-180 adult samples (~75-90 by species) and 25-30 groups of larvae from different nests. The Screening of potentially adaptive genetic variation will be performed using different approaches: a) Genomics seascape: We will use SamBada35 and related software to perform these analyses. b) Comparing allele frequencies of functional gene groups to neutral distributions and estimating statistics that are informative on the action of balancing selection. c) Random Forest algorithm can discover subtle changes in allele frequencies among environments than are undetectable using traditional population genetics approaches and we will use it as implemented in R packages. The deliverables of the project, as well as the designed pipelines for data analyses will be available for research and non-profit initiatives through the website of the project which will be created for this purpose within the institutional host web-service. Thus, the research proposal project will be connected to the DigitalCSIC data repository, which is also associated with other repositories (R3data, FAIRsharingData, etc) and Search platforms (Scholar google, etc) ensuring the correct dissemination and availability of the data, methods and results. Moreover, genomics sequences generated with this project will be deposit in public databases such as NCBI-bioproject database freely accessible for the whole scientific community.
Dissemination of the results of the project will be achieved by participating in scientific symposia and congresses, by peer-reviewed scientific publications, conferences, outreach talks, and by creating graphical material to distribute online through the web page of the host institutions and social media accounts. We have planned to participate in the following conferences, symposia and meetings, among others: ICES Annual Science Conference 2022 12-15 September 2022 Dublin, Ireland, where we will present Results of WP1 (D1); 5th International Symposium The Effects of Climate Change on the World’s Oceans. 17-21 April 2023 Bergen, Norway; where we will present the results of WP2 (D2); ASLO 2023 Aquatic Sciences Meeting. JUNE 4-9, 2023. Palma de Mallorca, Spain. where we will present the results of WP3 (D3); and ICES Annual Science Conference 2023 (~September); presenting results of WP4 (D4).The host institute, IIM-CSIC, has a strong commitment engaging Science with Society and a wide experience in education and outreach activities. The IIM-CSIC has its own Engagement with Society Unit (UCC+I) which works closely with the Scientific Culture Unit (UCC) of the CSIC's Delegation in Galicia, and will be able to give support to the dissemination and communication plan of this project through institutional web-pages and social media accounts. Moreover, the deliverables of the project will be available for research and non-profit initiatives through the IIM-CSIC website that is connected to the DigitalCSIC data repository, which is also associated with other repositories (R3data, FAIRsharingData, etc) and Search platforms (Scholar google, etc) ensuring the correct dissemination and availability of data and results.We will participate in outreach activities planned by the host institution and will produce graphical material to properly disseminate the FishAdapt project. Outreach activities are planned to be realized through the whole lifetime of the project fitting the Institution’s agenda. Outreach talks and graphical material will be addressed to different communities: 1) fishers: promoting sustainable fishing practices in relation to Climate Change adaptation; 2) governmental agencies and fisheries stakeholders: transferring the results and methodological approaches to influence decision-making policies in fisheries management; and 3) citizen in general as end-users/consumers: raising awareness on sustainable consumption of marine resources. Finally, the researcher candidate is committed to outreach and citizen sciences projects, and therefore she will disseminate the project goal and the research outputs through the lifetime of the project also through her personal twitter account @natpetit1.Given the high quality of the proposed research, we planned to submit two publications with the results to high-impact journals relevant to the research field such as Global Change Biology, Fish and Fisheries, and Molecular Ecology. The submission of the Research Output (RO1) “adaptive potential to environmental change in exploited fish” is planned to be before the end of the first year of work (nearly April-May 2023); while the submission of the RO2 is planned to be before the end of the second year (around April-May 2024). The two publications will be available first as pre-prints and then by open access publication, increasing their dissemination chances.
Publicaciones
Autores:
Natalia Petit-Marty; Laura Casas; Fran Saborido-Rey
Publicado en:
Frontiers in Marine Science, 2023, ISSN 2296-7745
Editor:
Frontiers
DOI:
10.3389/fmars.2023.1061530
Autores:
Francesca Ferragut-Perello; Sergio Ramírez-Amaro; Athanassios C. Tsikliras; Natalia Petit-Marty; Donna Dimarchopoulou; Enric Massutí; Alba Serrat; Francesc Ordines
Publicado en:
Fishes, 2023, ISSN 2410-3888
Editor:
MDPI
DOI:
10.3390/fishes8020117
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