Project description
Study of the early embryonic development at the individual cell level
Early embryonic development in mammals depends on spatiotemporal gene regulation at the individual cell level. Spatial transcriptomics at the single-cell level and in toto imaging are recently developed techniques, which might help to understand the developmental process better, linking cell motion with the cell state. Funded by the Marie Skłodowska-Curie Actions programme, the sc4DMap project aims to use spatial transcriptomics combined with in toto imaging technology to investigate early mammalian development events. The real-time tracking of cell dynamics and spatiotemporal gene expression profiles across the developing embryo will provide information about cell movement, interaction with other cells, regulation of gene expression and cell fate determination.
Objective
Mammalian early embryonic development requires exquisite spatiotemporal gene regulation at the level of individual cells. However, it is still unclear and remains a fundamental challenge how cell behaviours govern gastrulation and organogenesis in early embryos. Two techniques have recently been developed to investigate cell fate decisions in early mammalian development (spatial transcriptomics at single-cell level) or to study the dynamic transition from single cells to fully formed organisms (in toto imaging), and if only these two techniques could be combined would we be able to link cell motion with cell state and more completely understand developmental processes. There is precedence for combining live cell imaging with gene expression data in ascidians and annelids, but this has yet to be done, at scale, in more complex mammalian systems. To this end, the proposal aims to investigate early mammalian development by linking spatial transcriptomics and in toto imaging data. This will be the first combination of real-time tracking of cellular dynamics and spatiotemporal gene expression profiles across the developing embryo, thus providing insight into how cells move, interact with each other and how they regulate their own gene expression, ultimately revealing the fate that cells adopt. Accordingly, I will develop a novel integration framework and generate the first cellular-resolution mammalian embryo developmental gene expression map in four dimensions that reflects both cellular dynamics and spatiotemporal gene expression profiles at the single-cell level. Moreover, this work will pave the way for multi-omics integration, which has been increasingly explored but to date is confined to the single-cell space.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine embryology
- natural sciences biological sciences zoology invertebrate zoology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2021-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.