Project description
Advancing in vitro cardiac tissue models
Cardiac diseases constitute a leading cause of morbidity and mortality worldwide. Studying the mechanisms underlying these diseases requires faithful in vitro models. However, production of such models has been hampered by the complex tissue architecture and function of the myocardium. The EU-funded RHYTHM project proposes to develop a micro-engineered heart tissue, using hydrogels as the scaffold for growth and differentiation of seeded cardiac cells. The capacity of these hydrogels to support electrical cell stimulation is expected to generate in vitro functionally mature cardiac tissue which can be employed in various research and drug discovery applications.
Objective
In this project, a micro-engineered heart tissue (EHT) device that will advance the physiological relevance of current in vitro 3D cardiac tissue models will be developed. In moving towards this goal, this project will synthesize conductive guanosine-quadruplex (GQ) hydrogels and implement them as cell scaffolds in EHT devices. Key aspects of biomaterials design are the unique conductive and hierarchical 3D fibrous network and the good biocompatibility of GQ hydrogels, which will support cardiac cell culture. Upon integration in the EHT platform, the mentioned properties of GQ hydrogels will enable electrical stimulation of embedded cardiac cells under mild pacing voltages, thereby promoting the formation of morphologically and functionally mature cardiac tissue. GQ hydrogels will demonstrate high flexibility and potentially broad utility as a biocompatible and conductive biomaterial for in vitro cell experimentation. The outcomes of this project will expand the horizon of pre-clinical 3D cardiac tissue models, paving the way for future approaches to drug testing and personalized cardiovascular medicine.
Fields of science
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Funding Scheme
HORIZON-AG-UN - HORIZON Unit GrantCoordinator
7522 NB Enschede
Netherlands