Project description
Innovative fluorescence-lifetime microscopy for biomedical applications
Fluorescence-lifetime imaging microscopy (FLIM) is an imaging technique where the fluorophore fluorescence lifetime, rather than its intensity, is used for image creation. This powerful method has applications in biomedicine and industry, providing physicochemical parameters of cells, tissues and biomolecular interactions. The EU-funded EOFLIM project is developing an innovative FLIM detector that uses electro-optical elements as temporal gating units to overcome existing limitations in frame rate and sensitivity. The novel electro-optical FLIM detector will have frame rates beyond 100 Hz for megapixel images with single-molecule sensitivity. The potential of the new detector will be demonstrated in super-resolution FLIM applications using live cells and tissues, and in surgical applications for cancer diagnosis in brain biopsies.
Objective
Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful method for monitoring physicochemical parameters of cells and tissues, as well as biomolecular interactions, with applications in science, medicine, and industry. However, its use is currently limited due to technological constraints regarding frame rate and sensitivity.
We have developed a FLIM detector that enables highly sensitive, high-speed FLIM. It is based on the use of electro-optical elements as temporal gating units. Electro-optical FLIM (EOFLIM) enables frame rates beyond 100 Hz for megapixel images, single-molecule sensitivity, and high-resolution imaging (numerical aperture > 1.3).
We will demonstrate the innovation potential of our detector in two key applications: Super-resolution FLIM and Surgical FLIM. Super-resolution FLIM will be done in collaboration with Prof. Huser (Univ. of Bielefeld). We will apply our EOFLIM detector to super-resolution microscopy at high frame rates in live cells and tissues. Surgical FLIM will be done in collaboration with Prof Unterhuber (Medical Univ. of Vienna) and will aim at diagnosing cancerous tissue in brain biopsies. Super-resolution FLIM and Surgical FLIM will demonstrate the wide applicability of EOFLIM in research and medicine.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine surgery
- natural sciences physical sciences optics microscopy super resolution microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC-POC - HORIZON ERC Proof of Concept Grants
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2022-POC1
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1010 WIEN
Austria
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