Fast Electro-Optic Fluorescence Lifetime Microscopy (EOFLIM) for super-resolution microscopy and brain surgery.

Información del proyecto

EOFLIM

Identificador del acuerdo de subvención: 101069260

DOI

10.3030/101069260

Proyecto cerrado

Fecha de la firma de la CE 30 Junio 2022

Fecha de inicio 1 Julio 2022

Fecha de finalización 31 Diciembre 2023

Financiado con arreglo a

• European Research Council (ERC)

Coste total

Sin datos

Aportación de la UE

€ 150 000,00

Coordinado por

UNIVERSITAT WIEN
Austria

Periodic Reporting for period 1 - EOFLIM (Fast Electro-Optic Fluorescence Lifetime Microscopy (EOFLIM) for super-resolution microscopy and brain surgery.)

Período documentado: 2022-07-01 hasta 2023-12-31

Resumen del contexto y de los objetivos generales del proyecto

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful method for monitoring physicochemical parameters of cells and tissues, as well as biomolecular interactions, with applications in science, medicine, and industry.

Its use is often limited due to technological constraints regarding frame rate and sensitivity: Most commercial solutions are based on Time-Correlated Single Photon Counting detectors, which offer low noise, but require a scanning approach to imaging, which is slow. Recently, various wide-field detectors have come up, which offer high speed, but lack sensitivity or quantum efficiency.

The limitations mentioned above can be overcome using our unique solution of utilizing electro-optic modulators for full-field temporal gating (EOFLIM). This gating method is compatible with low-noise, highly efficient state-of-the-art camera technology, enabling single molecule sensitivity and high frame rates. We will use this new technology to realize fast superresolution fluorescence lifetime imaging, and will explore EOFLIM in the context of surgical guidance technology.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

We have optimized our setup to reach aberration-free performance compatible with super-resolution microscopy.

We have set up a working super-resolution FLIM system.

We have adapted our setup for experiments testing our system in the context of tumor detection for surgical guidance.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

Our superresolution FLIM setup shows a unique combination of spatio-temporal bandwidth and low noise. We could use our setup on various cellular samples, demonstrating super-resolution, live-cell compatability, as well as lifetime-based sensing and multiplexing.