Fast Electro-Optic Fluorescence Lifetime Microscopy (EOFLIM) for super-resolution microscopy and brain surgery.

Projektinformationen

EOFLIM

ID Finanzhilfevereinbarung: 101069260

DOI

10.3030/101069260

Projekt abgeschlossen

EK-Unterschriftsdatum 30 Juni 2022

Startdatum 1 Juli 2022

Enddatum 31 Dezember 2023

Finanziert unter

• European Research Council (ERC)

Gesamtkosten

Keine Daten

EU-Beitrag

€ 150 000,00

Koordiniert durch

UNIVERSITAT WIEN
Austria

Periodic Reporting for period 1 - EOFLIM (Fast Electro-Optic Fluorescence Lifetime Microscopy (EOFLIM) for super-resolution microscopy and brain surgery.)

Berichtszeitraum: 2022-07-01 bis 2023-12-31

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful method for monitoring physicochemical parameters of cells and tissues, as well as biomolecular interactions, with applications in science, medicine, and industry.

Its use is often limited due to technological constraints regarding frame rate and sensitivity: Most commercial solutions are based on Time-Correlated Single Photon Counting detectors, which offer low noise, but require a scanning approach to imaging, which is slow. Recently, various wide-field detectors have come up, which offer high speed, but lack sensitivity or quantum efficiency.

The limitations mentioned above can be overcome using our unique solution of utilizing electro-optic modulators for full-field temporal gating (EOFLIM). This gating method is compatible with low-noise, highly efficient state-of-the-art camera technology, enabling single molecule sensitivity and high frame rates. We will use this new technology to realize fast superresolution fluorescence lifetime imaging, and will explore EOFLIM in the context of surgical guidance technology.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

We have optimized our setup to reach aberration-free performance compatible with super-resolution microscopy.

We have set up a working super-resolution FLIM system.

We have adapted our setup for experiments testing our system in the context of tumor detection for surgical guidance.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

Our superresolution FLIM setup shows a unique combination of spatio-temporal bandwidth and low noise. We could use our setup on various cellular samples, demonstrating super-resolution, live-cell compatability, as well as lifetime-based sensing and multiplexing.