Description du projet
Une méthode innovante de diagnostic de la résistance aux antibiotiques
La résistance aux antimicrobiens (RAM) est responsable de plus d’un million de décès dans le monde. La RAM est associée à la mauvaise utilisation et à la surprescription d’antibiotiques. Le développement de méthodes de diagnostic rapide repose sur des cultures bactériennes chronophages et souvent inefficaces. Une nouvelle approche a été conçue sur la base d’une technologie développée par des recherches antérieures du CER pour isoler l’ARN bactérien en quelques minutes. Le projet ResisCHIP, financé par le CER, développera et validera ces outils combinés à un réseau de puces à ARN rapide à une résolution monomoléculaire. Il fournira un kit de diagnostic sur mesure, sans culture, capable d’identifier des milliers de gènes et d’agents pathogènes à partir d’un échantillon de sang en moins de deux heures, ce qui permettra de sélectionner rapidement le meilleur traitement.
Objectif
Drug-resistant infections are one of the greatest threats facing humanity. Antimicrobial resistance (AMR) causes over 700.000 deaths each year. Furthermore, AMR is associated to reduced quality of life, increased hospitalization periods and medical costs. Misuse and over-prescription of antibiotics for human or animal treatment are one of the drivers of AMR. At the same time, the development of new antibiotics is virtually nonexistent. Hence, developing fast diagnostic methods to identify the optimal treatment is key to effectively fight AMR and increase survival rates of patients encountering life-threatening infections. So far, diagnostics rely on bacterial cultures, leading to slow turnaround times, which are linked to the preventive administration of broad-spectrum antibiotics, often inefficient and leading to worse disease outcomes and spread of AMR. Novel approaches such as DNA PCR-based diagnostic panels or next generation sequencing methods have been recently proposed to address this issue. However, they are target a limited number of pathogens and genes or require expensive equipment and highly-trained personnel. Furthermore, they require a culture step to reach detection levels, usually lasting a few hours, that can be critical for serious conditions. Recently, using technology derived from ERC StG and PoC grants, we designed a method to concentrate, purify, and isolate bacterial RNA in just 5 minutes. Here, we seek to develop and validate these tools, combined with a rapid RNA chip array with single-molecule resolution, to provide a culture-free, fast, and highly sensitive alternative to bacterial infection diagnostics from direct blood samples. Once developed, ResisCHIP will provide a tailor-made diagnostic kit with the capacity to identify thousands of genes and pathogens from a blood sample in less than 2 hours, allowing the rapid selection of the best treatment, improving AMR stewardess, and becoming a significant breakthrough in AMR diagnostics.
Champ scientifique
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural sciencesbiological sciencesgeneticsDNA
- medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantibiotics
- natural sciencesbiological sciencesgeneticsRNA
- medical and health sciencesbasic medicinepharmacology and pharmacydrug resistanceantibiotic resistance
Programme(s)
- HORIZON.1.1 - European Research Council (ERC) Main Programme
Régime de financement
ERC-POC - Proof of Concept GrantInstitution d’accueil
08193 Cerdanyola Del Valles
Espagne