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BIOS: The bio-intelligent DBTL cycle, a key enabler catalysing the industrial transformation towards sustainable biomanufacturing

Project description

Utilising bio-intelligent DBTL cycles for improved innovation testing

A sustainable circular economy can protect the environment and mitigate the impacts of climate change by reducing waste and greenhouse gas emissions. A circular bioeconomy approach converts sustainable substrates in bioprocesses to provide a range of innovations. However, testing under industrial conditions for synthetic biology solutions is still proving difficult, leading to many innovations failing to make it to market. The EU-funded BIOS project will address this challenge by introducing a bio-intelligent design-build-test-learn cycle for maturing new synthetic biology innovations. It will utilise a mix of biological and mechanical technologies and methodologies to make reaching the final stage more efficient and easier.

Objective

The usage of fossil resources leading to increasing atmospheric CO2 levels and global climate change should be rapidly replaced by implementing a circular economy. Circular bioeconomy converting sustainable substrates in moderately operating bioprocesses offers a plenitude of solutions. While synthetic biology provides a multitude of tools for strain engineering, their rapid use in hosts for optimal performance under industrial conditions is still challenging. Promising innovations are often trapped in the valley-of-death as strain engineering faces a too complex space of putative manipulations. Novel approaches are needed to increase speed and success rate of strain and bioprocess engineering.
The bio-intelligent approach, rigorously applied in BIOS, aims to accelerate and improve the conventional design-build-test-learn (DBTL) cycle for strain and bioprocess engineering. Interdisciplinary collaboration will bridge microbiology, molecular biology, biochemical engineering with informatics, automation engineering, and mechanical engineering. Novel innovative metrics, biosensors, and bioactuators are developed for bi-directionally communication at biological-technical interfaces. Digital twins are created mimicking cellular and process levels. Integrating AI not only improves prediction quality but also enables hybrid learning, the key reason to increase speed and success rate in the novel bio-intelligent DBTL cycle (biDBTL). The power of biDBTL will be showcased by creating P. putida producer strains for terpenes, polyolefines, and methylacrylate. All are highly attractive products with a high potential for reducing anthropogenic greenhouse footprint. BIOS will open the door to a de-centralized, networked collaboration for strain and process engineering that efficiently links individual expertise for the sake of a symbiotic and rapid progress. BIOS also paves the way to de-centralized bio-manufacturing by implementing autonomous, self-controlled bioprocesses.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-RIA - HORIZON Research and Innovation Actions

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-CL4-2021-DIGITAL-EMERGING-01

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Coordinator

UNIVERSITY OF STUTTGART
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 512 806,00
Address
KEPLERSTRASSE 7
70174 Stuttgart
Germany

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Region
Baden-Württemberg Stuttgart Stuttgart, Stadtkreis
Activity type
Higher or Secondary Education Establishments
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 512 806,25

Participants (6)

Partners (2)

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