Project description DEENESFRITPL Embryo-like structures with blood vessels Gastruloids are multi-tissue embryonic organoids (miniaturised and simplified versions of an organ) that can replicate mammalian developmental processes, including early organogenesis, in a petri dish. They hold great promise for scientific advancement through their ability to model organs in their earliest recognisable stages. However, current organoids are small and lack the ability to develop their own vascular networks. Focusing on cardiovascular and foregut development, the EU-funded SUMO project applied cutting-edge technologies, including artificial intelligence and bioengineering to develop reproducible and scalable formation of gastruloids with embryo-like morphology that demonstrate an advanced stage of organ development. Show the project objective Hide the project objective Objective The lack of realistic in vitro organ models that can faithfully represent in vivo physiological processes is a major obstacle affecting the biological and medical sciences. The current gold standard is animal experiments, but it is increasingly clear that these models mostly fail to recapitulate the human physiology. Moreover, animal experiments are controversial, and it is a common goal in the scientific community to minimize the use of animals to a strictly necessary minimum. The emergence of stem cell engineered organ models called organoids represents the only viable alternative to animal research. However, current organoid technology is yet to produce the larger physiologically relevant organmodels that the medical sciences really need. Specifically, current organoids are too small, not vascularized and lack the 3-dimensional organization found in vivo. In this interdisciplinary project we aim to challenge all these limitations by using the recently developed gastruloid technology guided by cutting edge bioengineering and artificial intelligence. Gastruloids are formed by initiating the very early developmental processes and develops along a highly coordinated three axial process that closely resembles mammalian embryogenesis. Moreover, gastruloids can develop several organ precursors simultaneously and thus constitutes important improvements over conventional single-tissue organoids. To harvest the potential of gastruloid technology we will first implement large sequencing and imaging experiments to optimize the developmental trajectory of gastruloids for organ inductions. We will then build these datasets into a multimodal data matrix to identify gastruloid candidates for cardiovascular and foregut development. Specifically, we will identify candidates that show strong vasculogenesis as candidates for later vascularisation by anastomose with endothelial cells. Fields of science natural sciencescomputer and information sciencesartificial intelligencenatural sciencesbiological sciencesdevelopmental biologymedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicinephysiology Keywords gastruloids artificial intelligence closed loop system organotypic modelling microfluidic chips multimodal imaging raman spectroscopic imaging microfluidics interdisciplinary science Programme(s) HORIZON.3.1 - The European Innovation Council (EIC) Main Programme Topic(s) HORIZON-EIC-2021-PATHFINDERCHALLENGES-01-05 - Engineered Living Materials Call for proposal HORIZON-EIC-2021-PATHFINDERCHALLENGES-01 See other projects for this call Funding Scheme HORIZON-EIC - HORIZON EIC Grants Coordinator OSLO UNIVERSITETSSYKEHUS HF Net EU contribution € 1 194 765,00 Address Kirkeveien 166 tarnbygget 0450 Oslo Norway See on map Region Norge Oslo og Viken Oslo Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 100 490,00 Participants (4) Sort alphabetically Sort by Net EU contribution Expand all Collapse all Third-party Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure. UNIVERSITETET I OSLO Norway Net EU contribution € 140 078,00 Address Problemveien 5-7 0313 Oslo See on map Region Norge Oslo og Viken Oslo Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,96 MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Germany Net EU contribution € 935 573,00 Address Hofgartenstrasse 8 80539 Munchen See on map Region Bayern Oberbayern München, Kreisfreie Stadt Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,58 FORSCHUNGSVERBUND BERLIN EV Germany Net EU contribution € 455 777,00 Address Rudower chaussee 17 12489 Berlin See on map Region Berlin Berlin Berlin Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,95 TEL AVIV UNIVERSITY Israel Net EU contribution € 611 532,00 Address Ramat aviv 69978 Tel aviv See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,19 Partners (2) Sort alphabetically Sort by Net EU contribution Expand all Collapse all IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE United Kingdom Net EU contribution € 0,00 Address South kensington campus exhibition road SW7 2AZ London See on map Region London Inner London — West Westminster Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 UNIVERSITY OF GLASGOW United Kingdom Net EU contribution € 0,00 Address University avenue G12 8QQ Glasgow See on map Region Scotland West Central Scotland Glasgow City Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
