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Defusing mini-chromosomes—the Achilles’ heel of the wheat blast pandemic

Project description

Tackling wheat blast pandemic to safeguard food supply

The wheat blast pandemic poses a major threat to our food supply, having spread rapidly from Brazil to Southeast Asia and Africa in just 35 years. This fungus, Magnaporthe oryzae, is particularly dangerous due to a special mini-chromosome that makes it even more harmful. To fight this problem, the ERC-funded PANDEMIC project aims to understand how the fungus works and find ways to protect wheat crops without resorting to genetically modified organisms (GMOs). By shedding light on the genetic and biophysical intricacies of pathogen-host interactions, PANDEMIC will pioneer non-transgenic solutions for wheat resistance. Through a multi-disciplinary approach spanning genetics, structural biology, and gene editing, PANDEMIC strives to secure our agricultural future against this insidious menace. Ensuring success in fighting the pandemic means safeguarding global food security.

Objective

PANDEMIC is an ambitious high-risk/high-gain program to deliver an innovative pathogen-guided strategy for crop disease resistance. The wheat blast pandemic is a clear and present danger to global food security. It is caused by the blast fungus, Magnaporthe oryzae, which, after emerging in Brazil only ~35 years ago, has spread over the last 5 years to Southeast Asia and Africa. We discovered that all isolates of the pandemic wheat blast fungus belong to a single, asexual clonal lineage. These pandemic isolates carry a supernumerary mini-chromosome (mChr) of ~2 Mb, which encodes 19 secreted effector (virulence) proteins and renders the pandemic lineage highly virulent. We propose that this mChr is the Achilles’ heel of the pandemic fungus. In PANDEMIC, my team and I will target the mChr virulence properties by gene editing and engineering of disease resistance genes that target mChr-encoded effectors. The central hypothesis of this project is, that detailed knowledge about the biophysical properties of pathogen effector-host target interactions can guide bioengineering of non-transgenic disease resistance in wheat.

To achieve our goal, we will employ a multi-disciplinary approach combining genetics, structural biology, and gene editing. Specifically, we will pursue the following objectives:

1. GENETICS. Determine the mChr-encoded effectors conferring high virulence on the pandemic wheat blast fungus.

2. BIOPHYSICS. Define the biophysical properties of effector-host target interactions.

3. IMMUNITY. Apply CRISPR/Cas gene editing to deliver innovative, non-transgenic solutions for blast disease management.

At the completion of this project, my team and I will generate a detailed understanding of the biophysical properties of mChr-encoded effector-target protein complexes and deliver novel traits and non-transgenic solutions for developing resistance against the pandemic wheat blast fungus.

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2022-STG

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Host institution

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 499 999,00
Address
HOFGARTENSTRASSE 8
80539 MUNCHEN
Germany

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Region
Bayern Oberbayern München, Kreisfreie Stadt
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 499 999,00

Beneficiaries (1)

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