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CORDIS - Resultados de investigaciones de la UE
CORDIS

Immunopeptidomics-based Development of Next-Generation Bacterial mRNA Vaccines

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Data management plan in place (se abrirá en una nueva ventana)

Report on the creation of the initial version of the project's data management plan, describing the intended data to be generated, the storage and handling of this data with an eye to IP and ethics constraints, and it's long-term archival and/or (where appropriate and possible) public dissemination approach.

Report on epitopes and antigens identified for MTB and BCG (se abrirá en una nueva ventana)

Description of the performance of a miniaturized and automated procedure for immunopeptidomics sample preparation, including MHC Class I and II peptide complex immunoprecipitation in 96-wells using a positive pressure liquid handling device.

Report on the application of SaaS on immunopeptidomics results (se abrirá en una nueva ventana)

Description of the utility of applying the Search All, Select Subset statistical approach for false discovery rate estimation on (small) subsets of identifications coming from mass spectrometry based proteomics approaches, on immunopeptidomics data sets.

Report on a miniaturized and (semi-)automated pipeline for immunopeptidomics sample preparation (se abrirá en una nueva ventana)

Description of the performance of a miniaturized and automated procedure for immunopeptidomics sample preparation, including MHC Class I and II peptide complex immunoprecipitation in 96-wells using a positive pressure liquid handling device.

Dissemination and exploitation plan (se abrirá en una nueva ventana)

The plan describes the planned measures to maximize the impact of the project, including the dissemination and exploitation measures that are planned, and the target group(s) addressed. Regarding communication measures and public engagement strategy, the aim is to inform and reach out to society and show the activities performed, and the use and the benefits the project will have for citizens.

Report on improved immunopeptide separation ionization and fragmentation (se abrirá en una nueva ventana)

Assessment of peptide TMT derivatization to improve ionization and fragmentation of MHC Class I and II immunopeptides along with EThcD fragmentation. Evaluation of peptide pre-fractionation to reduce sample complexity, and novel nanoLC columns that should increase the number of identified immunopeptides.

Policy briefing 1 (se abrirá en una nueva ventana)

At the end of the 1st reporting period, a report (policy briefing) will be shared with the policy makers.

Report on a new version of MS²Rescore with an EThcD-TMT model and DeepLC (se abrirá en una nueva ventana)

Description of a new version of the MSRescore immunopeptidomics mass spectrometry data analysis algorithm, implementing DeepLC retention time prediction, and an updated prediction algorithm for the prediction of fragmentation spectra for peptides carrying TMT tags, and fragmented using the EThcD method.

Setup of a project website and logo (se abrirá en una nueva ventana)

Report on the availability of a project website and a logo for the project.

Report on immuno-ionbot, a version of ionbot capable of performing open modification searches on immunopeptidomics data (se abrirá en una nueva ventana)

Description of an updated verison of the ionbot algorithm, which will be fitted with the capability to handle non-tryptic peptides (including immunopeptides), alongside the use of immunopeptide fragmentation and retention time predictions coming from the MS²PIP and DeepLC predictors, respectively.

IP management plan in place (se abrirá en una nueva ventana)

Report on the IP management plan as described in the project's Grant Agreement, including conflict resolution approaches.

Publicaciones

Thunder-DDA-PASEF enables high-coverage immunopeptidomics and is boosted by MS2Rescore with MS2PIP timsTOF fragmentation prediction model (se abrirá en una nueva ventana)

Autores: David Gomez-Zepeda, Danielle Arnold-Schild, Julian Beyrle, Arthur Declercq, Ralf Gabriels, Elena Kumm, Annica Preikschat, Mateusz Krzysztof Łącki, Aurélie Hirschler, Jeewan Babu Rijal, Christine Carapito, Lennart Martens, Ute Distler, Hansjörg Schild, Stefan Tenzer
Publicado en: Nature Communications, Edición 15, 2024, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-46380-Y

TIMS<sup>2</sup>Rescore: A Data Dependent Acquisition-Parallel Accumulation and Serial Fragmentation-Optimized Data-Driven Rescoring Pipeline Based on MS<sup>2</sup>Rescore (se abrirá en una nueva ventana)

Autores: Arthur Declercq, Robbe Devreese, Jonas Scheid, Caroline Jachmann, Tim Van Den Bossche, Annica Preikschat, David Gomez-Zepeda, Jeewan Babu Rijal, Aurélie Hirschler, Jonathan R Krieger, Tharan Srikumar, George Rosenberger, Claudia Martelli, Dennis Trede, Christine Carapito, Stefan Tenzer, Juliane S Walz, Sven Degroeve, Robbin Bouwmeester, Lennart Martens, Ralf Gabriels
Publicado en: Journal of Proteome Research, 2025, ISSN 1535-3893
Editor: American Chemical Society (ACS)
DOI: 10.1021/ACS.JPROTEOME.4C00609

Alpha-galactosylceramide improves the potency of mRNA LNP vaccines against cancer and intracellular bacteria (se abrirá en una nueva ventana)

Autores: Sofie Meulewaeter, Ilke Aernout, Joke Deprez, Yanou Engelen, Margo De Velder, Lorenzo Franceschini, Karine Breckpot, Serge Van Calenbergh, Caroline Asselman, Katie Boucher, Francis Impens, Stefaan C. De Smedt, Rein Verbeke, Ine Lentacker
Publicado en: Journal of Controlled Release, Edición 370, 2025, ISSN 0168-3659
Editor: Elsevier BV
DOI: 10.1016/j.jconrel.2024.04.052

PTMVision: An Interactive Visualization Webserver for Post-translational Modifications of Proteins (se abrirá en una nueva ventana)

Autores: Simon Hackl, Caroline Jachmann, Mathias Witte Paz, Theresa Anisja Harbig, Lennart Martens, Kay Nieselt
Publicado en: Journal of Proteome Research, Edición 24, 2025, ISSN 1535-3893
Editor: American Chemical Society (ACS)
DOI: 10.1021/acs.jproteome.4c00679

Immunopeptidomics Mapping of Listeria monocytogenes T Cell Epitopes in Mice (se abrirá en una nueva ventana)

Autores: Adillah Gul, Lecia L. Pewe, Patrick Willems, Rupert Mayer, Fabien Thery, Caroline Asselman, Ilke Aernout, Rein Verbeke, Denzel Eggermont, Laura Van Moortel, Ellen Upton, Yifeng Zhang, Katie Boucher, Laia Miret-Casals, Hans Demol, Stefaan C. De Smedt, Ine Lentacker, Lilliana Radoshevich, John T. Harty, Francis Impens
Publicado en: Molecular &amp; Cellular Proteomics, Edición 23, 2024, ISSN 1535-9476
Editor: Elsevier BV
DOI: 10.1016/j.mcpro.2024.100829

MS<sup>2</sup>Rescore 3.0 Is a Modular, Flexible, and User-Friendly Platform to Boost Peptide Identifications, as Showcased with MS Amanda 3.0 (se abrirá en una nueva ventana)

Autores: Louise M. Buur, Arthur Declercq, Marina Strobl, Robbin Bouwmeester, Sven Degroeve, Lennart Martens, Viktoria Dorfer, Ralf Gabriels
Publicado en: Journal of Proteome Research, Edición 23, 2024, ISSN 1535-3893
Editor: American Chemical Society (ACS)
DOI: 10.1021/acs.jproteome.3c00785

Paneth cell TNF signaling induces gut bacterial translocation and sepsis (se abrirá en una nueva ventana)

Autores: Charlotte Wallaeys, Natalia Garcia-Gonzalez, Steven Timmermans, Jolien Vandewalle, Tineke Vanderhaeghen, Somara De Beul, Hester Dufoor, Melanie Eggermont, Elise Moens, Victor Bosteels, Riet De Rycke, Fabien Thery, Francis Impens, Serge Verbanck, Stefan Lienenklaus, Sophie Janssens, Richard S. Blumberg, Takao Iwawaki, Claude Libert
Publicado en: Cell Host &amp; Microbe, Edición 32, 2024, ISSN 1931-3128
Editor: Elsevier BV
DOI: 10.1016/j.chom.2024.08.007

Maximizing immunopeptidomics-based bacterial epitope discovery by multiple search engines and rescoring (se abrirá en una nueva ventana)

Autores: Patrick Willems, Fabien Thery, Laura Van Moortel, Margaux De Meyer, An Staes, Adillah Gul, Lyudmila Kovalchuke, Arthur Declercq, Robbe Devreese, Robbin Bouwmeester, Ralf Gabriels, Lennart Martens, Francis Impens
Publicado en: 2024
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.11.22.624860

Collisional cross-section prediction for multiconformational peptide ions with IM2Deep (se abrirá en una nueva ventana)

Autores: Robbe Devreese, Alireza Nameni, Arthur Declercq, Emmy Terryn, Ralf Gabriels, Francis Impens, Kris Gevaert, Lennart Martens, Robbin Bouwmeester
Publicado en: 2025
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.02.18.638865

MHCquant2 refines immunopeptidomics tumor antigen discovery (se abrirá en una nueva ventana)

Autores: Jonas Scheid, Steffen Lemke, Naomi Hoenisch-Gravel, Anna Dengler, Timo Sachsenberg, Arthur Declerq, Ralf Gabriels, Jens Bauer, Marcel Wacker, Leon Bichmann, Lennart Martens, Marissa L. Dubbelaar, Sven Nahnsen, Juliane S. Walz
Publicado en: 2024
Editor: Springer Science and Business Media LLC
DOI: 10.21203/rs.3.rs-5560023/v1

Open-source and FAIR Research Software for Proteomics (se abrirá en una nueva ventana)

Autores: Yasset Perez-Riverol, Wout Bittremieux, William S. Noble, Lennart Martens, Aivett Bilbao, Michael R. Lazear, Bjorn Grüning, Daniel S. Katz, Michael J. MacCoss, Chengxin Dai, Jimmy K. Eng, Robbin Bouwmeester, Michael Robert Shortreed, Enrique Audain, Timo Sachsenberg, Jeroen Van Goey, Georg Wallmann, Bo Wen, Lukas Käll, William E. Fondrie
Publicado en: 2024
Editor: American Chemical Society (ACS)
DOI: 10.26434/CHEMRXIV-2024-WF6DW

Bioinformatics Pipeline for Processing Single-Cell Data (se abrirá en una nueva ventana)

Autores: Arthur Declercq, Nina Demeulemeester, Ralf Gabriels, Robbin Bouwmeester, Sven Degroeve, Lennart Martens
Publicado en: Methods in Molecular Biology, Mass Spectrometry Based Single Cell Proteomics, 2024
Editor: Springer US
DOI: 10.1007/978-1-0716-3934-4_15

Current State of mRNA Vaccine Development Against <i>Mycobacterium tuberculosis</i> (se abrirá en una nueva ventana)

Autores: Ilke Aernout, Rein Verbeke, Stefaan C. De Smedt, Francis Impens, Ine Lentacker
Publicado en: Trends in mRNA Vaccine Research, 2024
Editor: Wiley
DOI: 10.1002/9783527838394.ch12

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