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CORDIS - Risultati della ricerca dell’UE
CORDIS

Immunopeptidomics-based Development of Next-Generation Bacterial mRNA Vaccines

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Data management plan in place (si apre in una nuova finestra)

Report on the creation of the initial version of the project's data management plan, describing the intended data to be generated, the storage and handling of this data with an eye to IP and ethics constraints, and it's long-term archival and/or (where appropriate and possible) public dissemination approach.

Report on epitopes and antigens identified for MTB and BCG (si apre in una nuova finestra)

Description of the performance of a miniaturized and automated procedure for immunopeptidomics sample preparation, including MHC Class I and II peptide complex immunoprecipitation in 96-wells using a positive pressure liquid handling device.

Report on the application of SaaS on immunopeptidomics results (si apre in una nuova finestra)

Description of the utility of applying the Search All, Select Subset statistical approach for false discovery rate estimation on (small) subsets of identifications coming from mass spectrometry based proteomics approaches, on immunopeptidomics data sets.

Report on a miniaturized and (semi-)automated pipeline for immunopeptidomics sample preparation (si apre in una nuova finestra)

Description of the performance of a miniaturized and automated procedure for immunopeptidomics sample preparation, including MHC Class I and II peptide complex immunoprecipitation in 96-wells using a positive pressure liquid handling device.

Dissemination and exploitation plan (si apre in una nuova finestra)

The plan describes the planned measures to maximize the impact of the project, including the dissemination and exploitation measures that are planned, and the target group(s) addressed. Regarding communication measures and public engagement strategy, the aim is to inform and reach out to society and show the activities performed, and the use and the benefits the project will have for citizens.

Report on improved immunopeptide separation ionization and fragmentation (si apre in una nuova finestra)

Assessment of peptide TMT derivatization to improve ionization and fragmentation of MHC Class I and II immunopeptides along with EThcD fragmentation. Evaluation of peptide pre-fractionation to reduce sample complexity, and novel nanoLC columns that should increase the number of identified immunopeptides.

Policy briefing 1 (si apre in una nuova finestra)

At the end of the 1st reporting period, a report (policy briefing) will be shared with the policy makers.

Report on a new version of MS²Rescore with an EThcD-TMT model and DeepLC (si apre in una nuova finestra)

Description of a new version of the MSRescore immunopeptidomics mass spectrometry data analysis algorithm, implementing DeepLC retention time prediction, and an updated prediction algorithm for the prediction of fragmentation spectra for peptides carrying TMT tags, and fragmented using the EThcD method.

Setup of a project website and logo (si apre in una nuova finestra)

Report on the availability of a project website and a logo for the project.

Report on immuno-ionbot, a version of ionbot capable of performing open modification searches on immunopeptidomics data (si apre in una nuova finestra)

Description of an updated verison of the ionbot algorithm, which will be fitted with the capability to handle non-tryptic peptides (including immunopeptides), alongside the use of immunopeptide fragmentation and retention time predictions coming from the MS²PIP and DeepLC predictors, respectively.

IP management plan in place (si apre in una nuova finestra)

Report on the IP management plan as described in the project's Grant Agreement, including conflict resolution approaches.

Pubblicazioni

Thunder-DDA-PASEF enables high-coverage immunopeptidomics and is boosted by MS2Rescore with MS2PIP timsTOF fragmentation prediction model (si apre in una nuova finestra)

Autori: David Gomez-Zepeda, Danielle Arnold-Schild, Julian Beyrle, Arthur Declercq, Ralf Gabriels, Elena Kumm, Annica Preikschat, Mateusz Krzysztof Łącki, Aurélie Hirschler, Jeewan Babu Rijal, Christine Carapito, Lennart Martens, Ute Distler, Hansjörg Schild, Stefan Tenzer
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-024-46380-Y

TIMS<sup>2</sup>Rescore: A Data Dependent Acquisition-Parallel Accumulation and Serial Fragmentation-Optimized Data-Driven Rescoring Pipeline Based on MS<sup>2</sup>Rescore (si apre in una nuova finestra)

Autori: Arthur Declercq, Robbe Devreese, Jonas Scheid, Caroline Jachmann, Tim Van Den Bossche, Annica Preikschat, David Gomez-Zepeda, Jeewan Babu Rijal, Aurélie Hirschler, Jonathan R Krieger, Tharan Srikumar, George Rosenberger, Claudia Martelli, Dennis Trede, Christine Carapito, Stefan Tenzer, Juliane S Walz, Sven Degroeve, Robbin Bouwmeester, Lennart Martens, Ralf Gabriels
Pubblicato in: Journal of Proteome Research, 2025, ISSN 1535-3893
Editore: American Chemical Society (ACS)
DOI: 10.1021/ACS.JPROTEOME.4C00609

Alpha-galactosylceramide improves the potency of mRNA LNP vaccines against cancer and intracellular bacteria (si apre in una nuova finestra)

Autori: Sofie Meulewaeter, Ilke Aernout, Joke Deprez, Yanou Engelen, Margo De Velder, Lorenzo Franceschini, Karine Breckpot, Serge Van Calenbergh, Caroline Asselman, Katie Boucher, Francis Impens, Stefaan C. De Smedt, Rein Verbeke, Ine Lentacker
Pubblicato in: Journal of Controlled Release, Numero 370, 2025, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2024.04.052

PTMVision: An Interactive Visualization Webserver for Post-translational Modifications of Proteins (si apre in una nuova finestra)

Autori: Simon Hackl, Caroline Jachmann, Mathias Witte Paz, Theresa Anisja Harbig, Lennart Martens, Kay Nieselt
Pubblicato in: Journal of Proteome Research, Numero 24, 2025, ISSN 1535-3893
Editore: American Chemical Society (ACS)
DOI: 10.1021/acs.jproteome.4c00679

Immunopeptidomics Mapping of Listeria monocytogenes T Cell Epitopes in Mice (si apre in una nuova finestra)

Autori: Adillah Gul, Lecia L. Pewe, Patrick Willems, Rupert Mayer, Fabien Thery, Caroline Asselman, Ilke Aernout, Rein Verbeke, Denzel Eggermont, Laura Van Moortel, Ellen Upton, Yifeng Zhang, Katie Boucher, Laia Miret-Casals, Hans Demol, Stefaan C. De Smedt, Ine Lentacker, Lilliana Radoshevich, John T. Harty, Francis Impens
Pubblicato in: Molecular &amp; Cellular Proteomics, Numero 23, 2024, ISSN 1535-9476
Editore: Elsevier BV
DOI: 10.1016/j.mcpro.2024.100829

MS<sup>2</sup>Rescore 3.0 Is a Modular, Flexible, and User-Friendly Platform to Boost Peptide Identifications, as Showcased with MS Amanda 3.0 (si apre in una nuova finestra)

Autori: Louise M. Buur, Arthur Declercq, Marina Strobl, Robbin Bouwmeester, Sven Degroeve, Lennart Martens, Viktoria Dorfer, Ralf Gabriels
Pubblicato in: Journal of Proteome Research, Numero 23, 2024, ISSN 1535-3893
Editore: American Chemical Society (ACS)
DOI: 10.1021/acs.jproteome.3c00785

Paneth cell TNF signaling induces gut bacterial translocation and sepsis (si apre in una nuova finestra)

Autori: Charlotte Wallaeys, Natalia Garcia-Gonzalez, Steven Timmermans, Jolien Vandewalle, Tineke Vanderhaeghen, Somara De Beul, Hester Dufoor, Melanie Eggermont, Elise Moens, Victor Bosteels, Riet De Rycke, Fabien Thery, Francis Impens, Serge Verbanck, Stefan Lienenklaus, Sophie Janssens, Richard S. Blumberg, Takao Iwawaki, Claude Libert
Pubblicato in: Cell Host &amp; Microbe, Numero 32, 2024, ISSN 1931-3128
Editore: Elsevier BV
DOI: 10.1016/j.chom.2024.08.007

Maximizing immunopeptidomics-based bacterial epitope discovery by multiple search engines and rescoring (si apre in una nuova finestra)

Autori: Patrick Willems, Fabien Thery, Laura Van Moortel, Margaux De Meyer, An Staes, Adillah Gul, Lyudmila Kovalchuke, Arthur Declercq, Robbe Devreese, Robbin Bouwmeester, Ralf Gabriels, Lennart Martens, Francis Impens
Pubblicato in: 2024
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.11.22.624860

Collisional cross-section prediction for multiconformational peptide ions with IM2Deep (si apre in una nuova finestra)

Autori: Robbe Devreese, Alireza Nameni, Arthur Declercq, Emmy Terryn, Ralf Gabriels, Francis Impens, Kris Gevaert, Lennart Martens, Robbin Bouwmeester
Pubblicato in: 2025
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.02.18.638865

MHCquant2 refines immunopeptidomics tumor antigen discovery (si apre in una nuova finestra)

Autori: Jonas Scheid, Steffen Lemke, Naomi Hoenisch-Gravel, Anna Dengler, Timo Sachsenberg, Arthur Declerq, Ralf Gabriels, Jens Bauer, Marcel Wacker, Leon Bichmann, Lennart Martens, Marissa L. Dubbelaar, Sven Nahnsen, Juliane S. Walz
Pubblicato in: 2024
Editore: Springer Science and Business Media LLC
DOI: 10.21203/rs.3.rs-5560023/v1

Open-source and FAIR Research Software for Proteomics (si apre in una nuova finestra)

Autori: Yasset Perez-Riverol, Wout Bittremieux, William S. Noble, Lennart Martens, Aivett Bilbao, Michael R. Lazear, Bjorn Grüning, Daniel S. Katz, Michael J. MacCoss, Chengxin Dai, Jimmy K. Eng, Robbin Bouwmeester, Michael Robert Shortreed, Enrique Audain, Timo Sachsenberg, Jeroen Van Goey, Georg Wallmann, Bo Wen, Lukas Käll, William E. Fondrie
Pubblicato in: 2024
Editore: American Chemical Society (ACS)
DOI: 10.26434/CHEMRXIV-2024-WF6DW

Bioinformatics Pipeline for Processing Single-Cell Data (si apre in una nuova finestra)

Autori: Arthur Declercq, Nina Demeulemeester, Ralf Gabriels, Robbin Bouwmeester, Sven Degroeve, Lennart Martens
Pubblicato in: Methods in Molecular Biology, Mass Spectrometry Based Single Cell Proteomics, 2024
Editore: Springer US
DOI: 10.1007/978-1-0716-3934-4_15

Current State of mRNA Vaccine Development Against <i>Mycobacterium tuberculosis</i> (si apre in una nuova finestra)

Autori: Ilke Aernout, Rein Verbeke, Stefaan C. De Smedt, Francis Impens, Ine Lentacker
Pubblicato in: Trends in mRNA Vaccine Research, 2024
Editore: Wiley
DOI: 10.1002/9783527838394.ch12

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