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A calorimeter at atomic resolution

Project description

Thermodynamics measurements at the atomic level

Molecular interactions between large and small molecules, such as proteins and drugs, are important for biological processes and for therapies. Molecular interactions are driven by the strength of the bonds formed between molecules (enthalpy) and the possible ways in which the molecules can interact (entropy). Funded by the European Research Council, the CLAR project aims to develop a new nuclear magnetic resonance (NMR) spectroscopy approach to measure thermodynamics within molecules and molecular complexes at atomic resolution. This method will help unveil the mechanisms underlying molecular function at the atomic level and is expected to find applications in drug design.

Objective

Molecular interactions are at the basis of all biological processes and often include specific interactions between macromolecules (protein, RNA, DNA) and small-molecule ligands, such as cofactors, hormones, drugs, or metabolites. Detailed and quantitative knowledge of these interactions is critical for a molecular understanding of these biological processes and developing new therapeutic solutions. A complete comprehension of molecular recognition requires a full characterization of the geometry and dynamics of the molecular complex. This includes not only the internal dynamics of each partner but also the dynamics at the interface, which to date remain mostly unexplored experimentally.
The physical chemistry frame for studying intra- and inter-molecular interactions is thermodynamics. The extent to which two molecules interact is dictated by the Gibbs energy change (G) of the interactions, which is composed of enthalpic (H) and entropic (S) terms.
X-ray crystallographic and NMR structures provide a detailed description of the static interactions associated with enthalpic contributions. However, up to now, the entropic components remain difficult to address experimentally.

The overarching goal of this proposal is to develop a calorimeter at atomic resolution.
To achieve that goal, a new NMR spectroscopy approach, relying mainly on the nuclear Overhauser effect, will be developed.

It is anticipated that quantitative thermodynamic measurements within molecules and molecular complexes will open a new avenue in the fundamental understanding of how atomistic mechanism(s) create a function.
Beyond the fundamental findings, we foresee applications in translational medicine, drug design, and computer-assisted molecular design.

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2022-COG

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Host institution

UNIVERSITAT WIEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 955 000,00
Address
UNIVERSITATSRING 1
1010 WIEN
Austria

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Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 955 000,00

Beneficiaries (1)

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