Rational and Simulation-Supported Design of Inhalable RNA Nanocarrier

Project Information

RatInhalRNA

Grant agreement ID: 101088587

DOI

10.3030/101088587

EC signature date 22 February 2023

Start date 1 April 2023

End date 31 March 2028

Funded under

• European Research Council (ERC)

Total cost

€ 2 000 000,00

EU contribution

€ 2 000 000,00

Coordinated by

LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (12)

Molecular Dynamics Simulations Elucidate the Molecular Organization of Poly(beta-amino ester) Based Polyplexes for siRNA Delivery

Author(s): Katharina M. Steinegger; Lars Allmendinger; Sebastian Sturm; Felix Sieber-Schäfer; Adrian P. E. Kromer; Knut Müller-Caspary; Benjamin Winkeljann; Olivia M. Merkel
Published in: Nano Letters, 2024, ISSN 1526-4602
Publisher: ACS Publications
DOI: 10.1021/ACS.NANOLETT.4C04291

Optimized lipid nanoparticles for pulmonary delivery of CRISPR/Cas9 targeting KRAS G12S in lung cancer

Author(s): Moritz Marschhofer, Siyu Chen, Müge Molbay, Benjamin Winkeljann, Ersilia Villano, Corinne Giancaspro, Alexandra Kourou, Otto Berninghausen, Susanne Rieder, Charlotte Ungewickell, Roland Beckmann, Bastian Popper, Ana Maria Torres, Anxo Vidal, Olivia M. Merkel, Simone P. Carneiro
Published in: Journal of Controlled Release, Issue 391, 2026, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/J.JCONREL.2026.114607

Synthesis and application of spermine-based amphiphilic poly(β-amino ester)s for siRNA delivery

Author(s): Yao Jin, Friederike Adams, Anny Nguyen, Sebastian Sturm, Simone Carnerio, Knut Müller-Caspary, Olivia M. Merkel
Published in: Nanoscale Advances, Issue 5, 2023, ISSN 2516-0230
Publisher: Royal Society of Chemistry (RSC)
DOI: 10.1039/D3NA00272A

Understanding excipient interactions unlocks untapped potential of RNA-lipid nanoparticles in dry powder formulations for local pulmonary delivery

Author(s): Nora Martini, Leonie Deßloch, Taras Sych, Otto Berninghausen, J. Merl-Pham, Sjoerd Dijkstra, Simone P. Carneiro, Marion Frankenberger, Roland Beckmann, Jürgen Behr, Gabriele Matschiner, Christine Schuberth-Wagner, A. Önder Yildirim, David C. Jürgens, Erdinc Sezgin, Olivia M. Merkel, Benjamin Winkeljann
Published in: Journal of Controlled Release, Issue 390, 2026, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/J.JCONREL.2025.114539

Machine Learning‐Enabled Polymer Discovery for Enhanced Pulmonary siRNA Delivery

Author(s): Felix Sieber-Schäfer, Min Jiang, Adrian Kromer, Anny Nguyen, Müge Molbay, Simone Pinto Carneiro, David Jürgens, Gerald Burgstaller, Bastian Popper, Benjamin Winkeljann, Olivia Monika Merkel
Published in: Advanced Functional materials, 2025, ISSN 1616-301X
Publisher: wiley
DOI: 10.1002/ADFM.202502805

Design of Experiments Grants Mechanistic Insights into the Synthesis of Spermine-Containing PBAE Copolymers

Author(s): Adrian P. E. Kromer; Felix Sieber-Schäfer; Johan Farfan Benito; Olivia M. Merkel
Published in: ACS Applied Materials & Interfaces, 2024, ISSN 1944-8252
Publisher: ACS Publications
DOI: 10.1021/ACSAMI.4C06079

Closing the Gap between Experiment and Simulation─A Holistic Study on the Complexation of Small Interfering RNAs with Polyethylenimine

Author(s): Jonas Binder, Joshua Winkeljann, Katharina Steinegger, Lara Trnovec, Daria Orekhova, Jonas Zähringer, Andreas Hörner, Valentin Fell, Philip Tinnefeld, Benjamin Winkeljann, Wolfgang Frieß, Olivia M. Merkel
Published in: Molecular Pharmaceutics, Issue 21, 2024, ISSN 1543-8384
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACS.MOLPHARMACEUT.3C00747

Nebulization of RNA-Loaded Micelle-Embedded Polyplexes as a Potential Treatment of Idiopathic Pulmonary Fibrosis

Author(s): Joschka T. Müller, Adrian P. E. Kromer, Aysan Ezaddoustdar, Ioannis Alexopoulos, Katharina M. Steinegger, Diana Leidy Porras-Gonzalez, Otto Berninghausen, Roland Beckmann, Peter Braubach, Gerald Burgstaller, Malgorzata Wygrecka, Olivia M. Merkel
Published in: ACS Applied Materials & Interfaces, Issue 17, 2025, ISSN 1944-8244
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSAMI.4C21657

Role of Hydrophobic Modification in Spermine-Based Poly(β-amino ester)s for siRNA Delivery and Their Spray-Dried Powders for Inhalation and Improved Storage

Author(s): Yao Jin; Xiaoxuan Wang; Adrian P.E. Kromer; Joschka T. Müller; Christoph Zimmermann; Zehua Xu; Achim Hartschuh; Friederike Adams; Olivia M. Merkel
Published in: Biomacromolecules, 2024, ISSN 1526-4602
Publisher: ACS Publications
DOI: 10.1021/ACS.BIOMAC.4C00283

A hybrid polymeric system for pulmonary mRNA delivery: Advancing mucosal vaccine development

Author(s): Min Jiang, Felix Sieber-Schäfer, Simone P. Carneiro, Dana Matzek, Anny Nguyen, Diana Leidy Porras-Gonzalez, Arun Kumar Verma, Miriam Kolog-Gulko, David C. Jürgens, Gerald Burgstaller, Bastian Popper, Xun Sun, Olivia M. Merkel
Published in: Cell Biomaterials, Issue 2, 2026, ISSN 3050-5623
Publisher: Elsevier BV
DOI: 10.1016/J.CELBIO.2025.100311

From Bits to Bonds: High-Throughput Virtual Screening of Ribonucleic Acid Nanocarriers Using a Combinatorial Approach of Machine Learning and Molecular Dynamics

Author(s): Felix Sieber-Schäfer, Jonas Binder, Tim Münchrath, Katharina M. Steinegger, Min Jiang, Benjamin Winkeljann, Wolfgang Friess, Olivia M. Merkel
Published in: Journal of the American Chemical Society, Issue 147, 2025, ISSN 0002-7863
Publisher: American Chemical Society (ACS)
DOI: 10.1021/JACS.5C12694

QbD product development: rapid optimization and scale-up of PBAE-based siRNA delivery <i>via</i> DoE-guided microfluidics

Author(s): Adrian P. E. Kromer, Laetitia J. M. Eller, David C. Jürgens, Olivia M. Merkel
Published in: RSC Pharmaceutics, 2026, ISSN 2976-8713
Publisher: Royal Society of Chemistry (RSC)
DOI: 10.1039/D5PM00379B

Other (1)

Endosomal escape mechanisms of extracellular vesicle-based drug carriers: lessons for lipid nanoparticle design

Author(s): Lasse Hagedorn; David C. Jürgens; Olivia M. Merkel; Benjamin Winkeljann
Published in: Extracellular Vesicles and Circulating Nucleic Acids, 2024, ISSN 2767-6641
Publisher: OAE Publishing Inc.
DOI: 10.20517/EVCNA.2024.19