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PHOtonic integrated OCT-enhanced flow cytometry for canceR and cardiovascular diagnostics enabled by Extracellular VEsicles discRimination

Project description

Multi-sensing platform for cancer and cardiovascular diagnostics

Extracellular vesicles (EVs) in the blood can provide information about the state of their cells of origin and the presence and progression of diseases. The EU-funded PHOREVER project will develop a multi-sensing platform to enable the detection of ultra-small EVs. The platform's operation will include flow cytometry (FCM) and AI-processing algorithms as the main method for EV detection and size classification as well as dual-channel swept-source optical coherence tomography with 2.5 µm resolution and fluorescence sensing for biomarker detection with increased accuracy. The medical impact of the platform will be evaluated in cases of pancreatic cancer with a focus on metastasis risk assessment, and in cases of stroke with a focus on fast and precise diagnosis.

Objective

Imaging tools such as the Computed Tomography (CT) and the Magnetic Resonance Imaging (MRI) can offer diagnosis of the cancer and the cardiovascular disease (CVD), but not insight into the molecular mechanisms that promote their occurrence, progression and possible resistance to treatment. Information about these mechanisms is present however in the blood. Extracellular vesicles (EVs) are secreted into the blood, and can inform us about the state of their cells of origin, and by extension, about the presence and progression of diseases. Unfortunately, their detection is still imperfect due to the ultra-small size (50-200 nm) of most of them. PHOREVER will develop a disruptive multi-sensing platform that will enable for the first time the reliable detection of EVs with size down to 80 nm, the detection of EVs with specific biomarkers (proteins) on their surface, and the calculation of the corresponding EV concentrations in the blood. Its operation will be based on 3 sensing modalities: Flow-cytometry (FCM) with 4 wavelengths (405, 488, 633 and 785 nm) as the main modality for EV detection and size classification, dual-channel swept-source optical coherence tomography (SS-OCT) with 2.5 µm resolution for imaging of the sensing area and noise reduction of the FCM measurements, and fluorescence sensing at 488 nm for biomarker detection after staining. The key components will be the 2 photonic integrated circuits in TriPleX and the 3 microfluidic chips, which will be integrated as a compact point-of-care device. The medical impact can be ground-breaking. The first use case will be related to pancreatic cancer with focus on progression monitoring, metastasis risk assessment, and treatment efficacy evaluation. The second use case will be related to stroke with focus on its fast and precise diagnosis for time-to-treatment reduction. In either case, data analysis empowered by artificial intelligence will correlate the measurement data to disease specific medical information.

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Topic(s)

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HORIZON-RIA - HORIZON Research and Innovation Actions

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Call for proposal

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(opens in new window) HORIZON-CL4-2022-DIGITAL-EMERGING-01

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Coordinator

EREVNITIKO PANEPISTIMIAKO INSTITOUTO SYSTIMATON EPIKOINONION KAI YPOLOGISTON
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 961 875,00
Address
PATISION 42
106 82 ATHINA
Greece

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 961 875,00

Participants (6)

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