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Architecture of Peripheral Neuroimmune Circuits and Synapses

Objective

Maintenance of health requires the coordination of multiple cellular networks. For example, the immune and nervous systems cooperate to regulate tissue homeostasis. In agreement, our recent work demonstrates that innate lymphoid cells (ILC) integrate neuronal signals to control tissue health and immunity. These findings are provoking a paradigm shift in our understanding of the immune response, neuroimmune crosstalk and its potential therapeutic value. Nevertheless, the dynamics of neuroimmune modalities remain elusive, and progress in the field has been hindered by the lack of approaches to explore the identity and plasticity of neuroimmune interactions in vivo.

Here, we hypothesise that dynamic circuitry codes orchestrate neuro-ILC2 interactions and disease outcomes. To test this hypothesis, we have developed a set of disruptive intercellular labelling neuroimmune toolboxes that we termed KISS and LIPSTIC, and which can probe the dynamics of neuro-ILC2 axes in vivo, with cellular specificity and single-cell resolution. Using these innovative platforms, we plan to unravel the architecture of pulmonary neuro-ILC2 circuits and to define cellular identities, outcomes, and plasticity at the neuroimmune interface. Sequentially, we propose to unravel unappreciated neuro-ILC2 synaptic communication and to define the ultrastructure of theses intercellular entities using high-resolution imaging. Finally, by conditionally harnessing the activity of synaptic neuronal partners during airway inflammation and infection, we will investigate their impact on ILC2, their environment, and on disease progression.

Together, these experiments will tackle multiple facets of pioneer, frontier questions, bringing to bear an array of cutting-edge technologies to address and advance, with unprecedented mechanistic and conceptual detail, how the neuroimmune interactome unfolds, in health and disease.

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Programme(s)

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Topic(s)

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2022-ADG

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Host institution

FUNDACAO D. ANNA DE SOMMER CHAMPALIMAUD E DR. CARLOS MONTEZ CHAMPALIMAUD
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 3 500 000,00
Address
AVENIDA BRASILIA, CENTRO DE INVESTIGACAO DA FUNDACAO CHAMPALIMAUD
1400-038 LISBOA
Portugal

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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 3 500 000,00

Beneficiaries (1)

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