Project description
Real-time imaging of stem cell differentiation
Light microscopy is routinely used in research and the diagnostic field around the world, enabling the visualisation of objects down to 250 nm in size. Super-resolution (SR) microscopy is gaining ground over optical microscopy as it bypasses the diffraction limit and significantly improves the resolution capacity. Funded by the European Innovation Council, the RT-SuperES project aims to develop an automated SR technology that offers the possibility of real-time imaging that can switch from conventional to SR fluorescence microscopy. Researchers plan to use this SR system to study the differentiation of embryonic stem cells.
Objective
The development of super-resolution (SR) microscopy in recent years has revolutionized cell biology, breaking the diffraction limit of light microscopy by order of magnitude. However, SR is currently incompatible with high-content imaging. RT-SuperES will provide a groundbreaking and affordable technology with automated SR capabilities beyond the state-of-the-art. To this end, we will generate a library of endogenously-labelled SNAP-tag fusion proteins in mouse embryonic stem cells (ESCs), and deploy a real-time decision-making module, which will continuously monitor our SNAP-tagged cells using fast fluorescence imaging, and, once a change is detected, will fix the desired cells, and switch to SR mode. By bringing together seven world-leading experts from four different countries, combining basic and applied research and industry, we propose several firsts: a) The first endogenously-labelled clone library of SNAP-tag fusion proteins; b) Utilize machine learning (ML) for real-time automated decision making, autonomously switching from fast conventional to SR imaging; c) Combine high content with SR imaging; d) Integrate novel, cutting-edge technologies, namely SR Radial Fluctuations (SRRF), NanoJ-Fluidics, Single Molecule Localization Microscopy (SMLM) and Structured Illumination Microscopy (SIM); e) Collect large scale imaging datasets of cell states in ESCs, and f) Provide cell-cycle stage-dependent nanoscale localization of selected nuclear and chromatin proteins (e.g. H3.3) during early ESC differentiation. RT-SuperES will provide the scientific community with the first-of-its-kind commercial real-time SR-highcontent imaging system, and the first library of endogenously SNAP-tagged ESC clones, which are ideal, among many other things, for SR imaging.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences biochemistry biomolecules proteins
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
HORIZON.3.1 - The European Innovation Council (EIC)
MAIN PROGRAMME
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-EIC - HORIZON EIC Grants
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-EIC-2022-PATHFINDEROPEN-01
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
91904 JERUSALEM
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.