Real-Time high-content Super-Resolution Imaging of ES Cell States

Información del proyecto

RT-SuperES

Identificador del acuerdo de subvención: 101099654

DOI

10.3030/101099654

Fecha de la firma de la CE 22 Diciembre 2022

Fecha de inicio 1 Julio 2023

Fecha de finalización 30 Junio 2027

Financiado con arreglo a

• The European Innovation Council (EIC)

Coste total

€ 3 488 483,13

Aportación de la UE

€ 3 488 483,00

Coordinado por

THE HEBREW UNIVERSITY OF JERUSALEM
Israel

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (8)

Methodology and analysis pipeline for SNAP-STORM imaging of histones

Characterise histone distribution in ESCs by SNAP-tag tools

Public engagement, new-media and outreach activities - midterm

Communication and new-media activities Outreach activities to youth and the wider public.

Validation of first SNAP-tag clones

Validate selected SNAP-TAG clones using SNAP-STORM imaging

Partial library of SNAP-tag proteins in ESCs

Generation of the first batch of endogenously-tagged SNAP-tag fusion protein library

Software and microfluidics design

Optimise the SRRF algorithm and NanoJ-Fluidics for the generated ESC libraries.Adopt (from WP2) and train the ML classifiers to interpret cellular states present in the library

Methodology and analysis pipeline for fixed 2-colour SNAP-STORM

Characterise the histone distribution using a 2-colour SNAP-STORM pipeline for fixed samples and comparison of the histone distribution in male and female ESCs

Website and project logo

Website and project logo information with also social media links

Verification of protocol for SNAP-tagging

Create a SNAP-TAG fusion protein library in ESCs

Data Management Plan (3)

RP1 update of the Data Management Plan

DMP for RP1

RP2 update of the Data Management Plan

DMP for RP2

Data Management Plan (DMP)

DMP monitoring

Publicaciones

Peer reviewed articles (13)

Structural Repetition Detector for multi-scale quantitative mapping of molecular complexes through microscopy

Autores: Afonso Mendes, Bruno M. Saraiva, Guillaume Jacquemet, João I. Mamede, Christophe Leterrier, Ricardo Henriques
Publicado en: Nature Communications, Edición 16, 2025, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-60709-1

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy

Autores: Daniel Krentzel, Matouš Elphick, Marie-Charlotte Domart, Christopher J. Peddie, Romain F. Laine, Cameron Shand, Ricardo Henriques, Lucy M. Collinson, Martin L. Jones
Publicado en: Nature Methods, Edición 22, 2025, ISSN 1548-7091
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41592-025-02794-0

Nature Communications

Autores: Joron, Khalil; Viegas, Juliane Oliveira; Haas-Neill, Liam; Bier, Sariel; Dvir, Shani; Lim, Siang Lin; Rauscher, Sarah; Meshorer, Eran; Lerner, Eitan
Publicado en: Nature Communications, 2023, ISSN 2041-1723
Editor: Nature Portfolio
DOI: 10.5281/zenodo.7964824

Efficiently accelerated bioimage analysis with NanoPyx, a Liquid Engine-powered Python framework

Autores: Bruno M. Saraiva, Inês Cunha, António D. Brito, Gautier Follain, Raquel Portela, Robert Haase, Pedro M. Pereira, Guillaume Jacquemet, Ricardo Henriques
Publicado en: Nature Methods, Edición 22, 2025, ISSN 1548-7091
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41592-024-02562-6

Nature Communications

Autores: Arfè, S.; Karagyozova, T.; Forest, A.; Bingham, D.; Hmidan, H.; Mazaud, D.; Garnier, M.; Le Baccon, P.; Meshorer, E.; Quivy, J.-P.; Almouzni, G.
Publicado en: Nature Communications, 2025, ISSN 2041-1723
Editor: Nature Communications
DOI: 10.1038/S41467-025-60430-Z

Harnessing artificial intelligence to reduce phototoxicity in live imaging

Autores: Estibaliz Gómez-de-Mariscal; Mario Del Rosario; Joanna W. Pylvänäinen; Guillaume Jacquemet; Ricardo Henriques
Publicado en: J Cell Sci, Edición (2024) 137 (3): jcs261545, 2024, ISSN 1477-9137
Editor: The Company of Biologists Ltd
DOI: 10.48550/arxiv.2308.04387

PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments

Autores: Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M. Pereira, Ricardo Henriques
Publicado en: Nature Communications, 2025, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-66209-6

Fibroblasts generate topographical cues that steer cancer cell migration

Autores: Francesco Baschieri, Abigail Illand, Jorge Barbazan, Olivier Zajac, Clémence Henon, Damarys Loew, Florent Dingli, Danijela Matic Vignjevic, Sandrine Lévêque-Fort, Guillaume Montagnac
Publicado en: SCIENCE ADVANCES, Edición Vol 9, Edición 33, 2023, ISSN 2375-2548
Editor: AAAS
DOI: 10.1126/sciadv.ade2120

The rise of data-driven microscopy powered by machinelearning

Autores: Leonor Morgado; Estibaliz Gómez-de-Mariscal; Hannah S. Heil; Ricardo Henriques
Publicado en: Journal of Microscopy, ISSN 0022-2720
Editor: Blackwell Publishing Inc.
DOI: 10.1111/JMI.13282

Nano-org, a functional resource for single-molecule localisation microscopy data

Autores: S. Shirgill, D. J. Nieves, J. A. Pike, M. A. Ahmed, H. Abbott, M. H. H. Baragilly, K. Savoye, J. D. Worboys, K. S. Hazime, E. Bruggeman, A. Garcia, D. J. Williamson, P. Rubin-Delanchy, R. Peters, D. M
Publicado en: Nature Communications, Edición 16, 2025, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-63674-X

HIRA defines early replication initiation zones independently of their genome compartment

Autores: Tina Karagyozova, Alberto Gatto, Audrey Forest, Jean-Pierre Quivy, Rocío Nunez-Vazquez, Marc A. Martí-Renom, Leonid A. Mirny, Geneviève Almouzni
Publicado en: Nature Communications, Edición 16, 2025, ISSN 2041-1723
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-65130-2

Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues

Autores: Dominik Kylies, Hannah S. Heil, Arturo G. Vesga, Mario Del Rosario, Maria Schwerk, Malte Kuehl, Milagros N. Wong, Victor G. Puelles, Ricardo Henriques
Publicado en: Nature Protocols, 2025, ISSN 1754-2189
Editor: Springer Science and Business Media LLC
DOI: 10.1038/S41596-025-01178-0

Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

Autores: Khalil Joron; Juliane Oliveira Viegas; Liam Haas-Neill; Sariel Bier; Paz Drori; Shani Dvir; Patrick Siang Lin Lim; Sarah Rauscher; Eran Meshorer; Eitan Lerner
Publicado en: Nature Communications, 2023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1101/2023.01.12.523769