Real-Time high-content Super-Resolution Imaging of ES Cell States

Informazioni relative al progetto

RT-SuperES

ID dell’accordo di sovvenzione: 101099654

DOI

10.3030/101099654

Data della firma CE 22 Dicembre 2022

Data di avvio 1 Luglio 2023

Data di completamento 30 Giugno 2027

Finanziato da

• The European Innovation Council (EIC)

Costo totale

€ 3 488 483,13

Contributo UE

€ 3 488 483,00

Coordinato da

THE HEBREW UNIVERSITY OF JERUSALEM
Israel

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (8)

Methodology and analysis pipeline for SNAP-STORM imaging of histones

Characterise histone distribution in ESCs by SNAP-tag tools

Public engagement, new-media and outreach activities - midterm

Communication and new-media activities Outreach activities to youth and the wider public.

Validation of first SNAP-tag clones

Validate selected SNAP-TAG clones using SNAP-STORM imaging

Partial library of SNAP-tag proteins in ESCs

Generation of the first batch of endogenously-tagged SNAP-tag fusion protein library

Software and microfluidics design

Optimise the SRRF algorithm and NanoJ-Fluidics for the generated ESC libraries.Adopt (from WP2) and train the ML classifiers to interpret cellular states present in the library

Methodology and analysis pipeline for fixed 2-colour SNAP-STORM

Characterise the histone distribution using a 2-colour SNAP-STORM pipeline for fixed samples and comparison of the histone distribution in male and female ESCs

Website and project logo

Website and project logo information with also social media links

Verification of protocol for SNAP-tagging

Create a SNAP-TAG fusion protein library in ESCs

Data Management Plan (3)

RP1 update of the Data Management Plan

DMP for RP1

RP2 update of the Data Management Plan

DMP for RP2

Data Management Plan (DMP)

DMP monitoring

Pubblicazioni

Peer reviewed articles (13)

Structural Repetition Detector for multi-scale quantitative mapping of molecular complexes through microscopy

Autori: Afonso Mendes, Bruno M. Saraiva, Guillaume Jacquemet, João I. Mamede, Christophe Leterrier, Ricardo Henriques
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-60709-1

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy

Autori: Daniel Krentzel, Matouš Elphick, Marie-Charlotte Domart, Christopher J. Peddie, Romain F. Laine, Cameron Shand, Ricardo Henriques, Lucy M. Collinson, Martin L. Jones
Pubblicato in: Nature Methods, Numero 22, 2025, ISSN 1548-7091
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41592-025-02794-0

Nature Communications

Autori: Joron, Khalil; Viegas, Juliane Oliveira; Haas-Neill, Liam; Bier, Sariel; Dvir, Shani; Lim, Siang Lin; Rauscher, Sarah; Meshorer, Eran; Lerner, Eitan
Pubblicato in: Nature Communications, 2023, ISSN 2041-1723
Editore: Nature Portfolio
DOI: 10.5281/zenodo.7964824

Efficiently accelerated bioimage analysis with NanoPyx, a Liquid Engine-powered Python framework

Autori: Bruno M. Saraiva, Inês Cunha, António D. Brito, Gautier Follain, Raquel Portela, Robert Haase, Pedro M. Pereira, Guillaume Jacquemet, Ricardo Henriques
Pubblicato in: Nature Methods, Numero 22, 2025, ISSN 1548-7091
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41592-024-02562-6

Nature Communications

Autori: Arfè, S.; Karagyozova, T.; Forest, A.; Bingham, D.; Hmidan, H.; Mazaud, D.; Garnier, M.; Le Baccon, P.; Meshorer, E.; Quivy, J.-P.; Almouzni, G.
Pubblicato in: Nature Communications, 2025, ISSN 2041-1723
Editore: Nature Communications
DOI: 10.1038/S41467-025-60430-Z

Harnessing artificial intelligence to reduce phototoxicity in live imaging

Autori: Estibaliz Gómez-de-Mariscal; Mario Del Rosario; Joanna W. Pylvänäinen; Guillaume Jacquemet; Ricardo Henriques
Pubblicato in: J Cell Sci, Numero (2024) 137 (3): jcs261545, 2024, ISSN 1477-9137
Editore: The Company of Biologists Ltd
DOI: 10.48550/arxiv.2308.04387

PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments

Autori: Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M. Pereira, Ricardo Henriques
Pubblicato in: Nature Communications, 2025, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-66209-6

Fibroblasts generate topographical cues that steer cancer cell migration

Autori: Francesco Baschieri, Abigail Illand, Jorge Barbazan, Olivier Zajac, Clémence Henon, Damarys Loew, Florent Dingli, Danijela Matic Vignjevic, Sandrine Lévêque-Fort, Guillaume Montagnac
Pubblicato in: SCIENCE ADVANCES, Numero Vol 9, Numero 33, 2023, ISSN 2375-2548
Editore: AAAS
DOI: 10.1126/sciadv.ade2120

The rise of data-driven microscopy powered by machinelearning

Autori: Leonor Morgado; Estibaliz Gómez-de-Mariscal; Hannah S. Heil; Ricardo Henriques
Pubblicato in: Journal of Microscopy, ISSN 0022-2720
Editore: Blackwell Publishing Inc.
DOI: 10.1111/JMI.13282

Nano-org, a functional resource for single-molecule localisation microscopy data

Autori: S. Shirgill, D. J. Nieves, J. A. Pike, M. A. Ahmed, H. Abbott, M. H. H. Baragilly, K. Savoye, J. D. Worboys, K. S. Hazime, E. Bruggeman, A. Garcia, D. J. Williamson, P. Rubin-Delanchy, R. Peters, D. M
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-63674-X

HIRA defines early replication initiation zones independently of their genome compartment

Autori: Tina Karagyozova, Alberto Gatto, Audrey Forest, Jean-Pierre Quivy, Rocío Nunez-Vazquez, Marc A. Martí-Renom, Leonid A. Mirny, Geneviève Almouzni
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41467-025-65130-2

Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues

Autori: Dominik Kylies, Hannah S. Heil, Arturo G. Vesga, Mario Del Rosario, Maria Schwerk, Malte Kuehl, Milagros N. Wong, Victor G. Puelles, Ricardo Henriques
Pubblicato in: Nature Protocols, 2025, ISSN 1754-2189
Editore: Springer Science and Business Media LLC
DOI: 10.1038/S41596-025-01178-0

Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation

Autori: Khalil Joron; Juliane Oliveira Viegas; Liam Haas-Neill; Sariel Bier; Paz Drori; Shani Dvir; Patrick Siang Lin Lim; Sarah Rauscher; Eran Meshorer; Eitan Lerner
Pubblicato in: Nature Communications, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1101/2023.01.12.523769