Description du projet
Profilage des interactions ARN-protéines dans l’infection par le virus respiratoire syncytial
Le virus respiratoire syncytial (VRS) touche environ 64 millions de personnes par an, causant plus de 150 000 décès. Les enfants de moins de cinq ans et les personnes âgées sont plus vulnérables à cette grave pathologie. Malgré son impact considérable sur la santé publique, la mise au point de vaccins et d’antiviraux s’est avérée compliquée en raison de la complexité des mécanismes de multiplication du virus, de ses interactions avec l’hôte et de sa pathogénicité. Le projet PARIS, soutenu par le programme Actions Marie Skłodowska-Curie, se propose de cartographier de manière exhaustive les interactions entre le VRS et l’hôte, en particulier les interactions ARN-protéines, et à caractériser la réponse cellulaire à l’infection. Pour ce faire, l’équipe s’appuiera sur une approche protéomique et transcriptomique intégrée dans des paradigmes expérimentaux similaires à une infection naturelle.
Objectif
Respiratory Syncytial Virus (RSV) is a major cause of severe lower respiratory tract infections and a major cause of mortality in children under 5 years old and the elderly worldwide. While RSV impose a huge disease burden on public healthcare system, the development of effective vaccines and specific antivirals is impeded by a lack of knowledge of virus multiplications mechanisms, virus-host interactions or pathogenicity. Here, we propose to comprehensively map RSV-host interactions and characterize the cellular response to infection through an integrated multi-omics approach and using experimental systems close to natural infection. This project will combine my expertise in RSV biology and the host laboratory’s profound experience in systems biology analysis. By using state-of-the-art-of proteomics and transcriptomics, we will characterize the RSV RNA-bound proteome as well as the protein-protein interactome, analyze the proteome of cells expressing the individual viral proteins (effectome), and assess the influence of RSV infection on cellular mRNA expression, protein abundance, and phosphorylation. We will systematically map the identified viral-induced perturbations onto a network of known cellular protein-protein interactions and signalling pathways. This comprehensive network will highlight RSV-induced perturbations and pinpoint RSV host dependency-factors, whose functional importance and therapeutic potential will be assessed by conducting genetic and drug-based loss and gain of function experiments. Biochemical and structural methods, and further analyses of the cellular response in genetically or drug-perturbed cells will yield insights into the molecular function of selected proteins. The proposed multilevel approach will thus reveal yet unstudied proteins and pathways determining RSV growth, pathology or viral host defense mechanisms, which will shed light on unresolved questions of RSV biology, and reveal hotspots amenable to therapeutic intervention.
Champ scientifique
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinateur
80333 Muenchen
Allemagne