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Supramolecular microcapsules for bioreactor expansion of induced pluripotent stem cells

Project description

Advancing stem cell culture technology

The in vitro culture of induced pluripotent stem cells (iPSCs) requires essential nutrients and growth factors to support their growth and proliferation. Funded by the European Research Council, the SUPROTECT project aims to develop microcapsules from squaramides, organic compounds with a unique chemical structure. Squaramides have been widely studied for their ability to form hydrogen bonds and complexes with a variety of molecules, including organic substrates and metal ions, leading to applications in catalysis, molecular recognition, and materials science. SUPROTECT will produce squaramide-based materials and test them for the 3D culture of iPSCs. Results will be consolidated for potential product licensing, advancing stem cell culture technology.

Objective

The SUPROTECT proof-of-concept project seeks to apply our squaramide-based (SQ) supramolecular materials to prepare microcapsules for the culture of pluripotent stem cells. Building on earlier demonstration of bioactive hydrogel synthesis and in vitro culture of iPSCs in 3D cell culture under static conditions, we will synthesize the necessary amount of material for iPSC culture studies, develop protocols to prepare microcapsules with and without iPSCs, and evaluate the feasibility of the different microcapsules for the culture of iPSCs and their release, ranking the approaches on iPSC number and quality for further downstream application. In parallel with these activities, we will perform detailed market research studies on microfabricated polymer materials for culture of various cell types, protecting our intellectual property (IP) position and consolidating our findings in a package for product licensing with the appropriate party.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-ERC-POC - HORIZON ERC Proof of Concept Grants

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Call for proposal

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(opens in new window) ERC-2023-POC

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Host institution

UNIVERSITEIT LEIDEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 150 000,00
Address
RAPENBURG 70
2311 EZ Leiden
Netherlands

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Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data

Beneficiaries (1)

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