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Deciphering Cancer Heterogeneity and Drug resistance using Single-Clone Genomic and Epigenomic Landscapes

Objective

To comprehend the characteristics, phylogenetic relationships, and drug resistance of tumor subclones, measurements must be performed at single-cell or single-clone resolution. Aims 1 and 2 aim to create cost-effective and reliable single-cell drop technologies for capturing full-length RNA (Scfl-RNA-Seq) and enhancers at the DNA level (ChromOpen). It is noteworthy that Scfl-RNA-Seq also captures non-coding and non-polyadenylated RNAs besides coding mRNA. ChromOpen also offers better capturing of functional enhancers and higher sensitivity compared to the conventional technology. The ultimate goal of Aim 3 is to create the first technology that can capture both full-length RNA and enhancers from the same cell (Multi-Omics). To increase sensitivity and coverage, Multi-Omics will be combined with a 3D-culturing system (3Dclone) that facilitates growth of primary cancer cells into small clones. The single-clone based Multi-Omics maps will increase the discovery rate of genetic somatic diversity, including non-coding regions, reveal connections between gene expression and mutated loci, and display a wider range of functional state associations between cells. In Aim 4, using our innovative mapping technologies, we will investigate two brain tumors: Non-Small Cell Lung Cancer that has spread to the brain and Glioblastoma primary cells. Both of these cancers have limited treatment options and poor outcomes due to drug resistance. Based on temporal drug treatment experiments and advanced mapping technologies, this data will improve our understanding of somatic driver mutations and cellular diversity in the context of resistance, and ultimately, lead to the identification of new therapeutic targets.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2023-COG

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Host institution

THE HEBREW UNIVERSITY OF JERUSALEM
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 000 000,00
Address
EDMOND J SAFRA CAMPUS GIVAT RAM
91904 JERUSALEM
Israel

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Activity type
Higher or Secondary Education Establishments
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 000 000,00

Beneficiaries (1)

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