Objective
Gas fermentation provides a unique opportunity for the circular bioeconomy by enabling carbon recycling from gaseous waste feedstocks into value-added bioproducts using microbes. Acetogens are ideal biocatalysts for gas fermentation as they use gas (CO and/or CO2+H2) as their sole carbon and energy source, evidenced by the commercialisation of the waste gas-to-ethanol conversion process. Despite recent efforts, our understanding of genotype-phenotype relationships in acetogens is still minimal. This, however, is required for rational metabolic engineering and expanding the product spectrum of acetogens to unlock their full potential. GENESYS aims to engineer superior gas-fermenting cell factories through revolutionising systems-level understanding of acetogens and pioneering a new workflow for making large-scale arrayed CRISPR-engineered microbial strain libraries. First, essential genes for autotrophy of the model acetogen Clostridium autoethanogenum will be determined using a genome-wide CRISPRi screen. We then create ~750 single-gene knock-down strains of essential genes, transcription factors, and proteome “dark matter” using a novel “pooled-to-arrayed” workflow combining synthetic biology, automation, and DNA sequencing. Next, we will generate systems-level phenotype maps for up to ~750 strains through high-throughput gas fermentation and –omics analyses. Big data will be translated into a systems-level acetogen knowledgebase–A-BASE–through integrated bioinformatics and machine learning, metabolic modelling, and data engineering. Finally, we apply A-BASE to engineer acetogen cell factories from the “unexplored territories” of essential genes, transcription factors, and proteome “dark matter” for improving production of ethanol and acetone. This project will create superior gas-fermenting cell factories, advance fundamental understanding of acetogen metabolism, and pioneer a new workflow for creating large-scale arrayed engineered microbial strain libraries.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences organic chemistry alcohols
- natural sciences chemical sciences catalysis biocatalysis
- engineering and technology industrial biotechnology biomaterials
- engineering and technology industrial biotechnology bioprocessing technologies fermentation
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-ERC - HORIZON ERC Grants
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) ERC-2023-COG
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
51005 Tartu
Estonia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.