Project description
Reliable, cost-effective and ultrasensitive detection of modified histones
Histones are proteins that attach to the DNA (chromosomes) in the nucleus and help to package and organise it. Once in the cytoplasm, post-translational modifications (PTMs) to histones are essential for many cellular processes including transcription and DNA repair. Understanding histone PTMs is important to preventing and treating diseases including cancer. Mass spectrometry (MS) relying on an expensive enrichment step that lacks reproducibility is commonly used to identify histone PTMs across the proteome. With the support of the Marie Skłodowska-Curie Actions programme, the ENRICH project aims to use functionalised nanoparticles to enrich PTM-containing peptides obtained with proteolytic digestion for subsequent MS analysis to overcome current limitations.
Objective
Scientific studies indicate that inefficient epigenetic control is associated with a wide variety of non-communicable diseases (NCDs) like cancer, schizophrenia, and diabetes. Indeed, histone post translational modifications (PTMs) are crucial for many cellular processes including transcription and DNA repair. Thus, the ability to readily and reliably detect PTMs is crucial to better understand epigenetic processes and the complex functions of histone PTMs in human diseases. Mass spectrometry (MS) is the technique of choice to identify such modifications across the proteome. MS requires an enrichment step generally performed using antibodies, but these have several limitations such as high costs, batch-to-batch variability and data reproducibility. In a multidisciplinary effort ENRICH aims at developing new cost-effective, fast and efficient tools for the enrichment of post translationally modified proteins overcoming the current limitation. ENRICH will functionalize nanoparticles (NPs) with molecular receptors able to enrich PTM-containing peptides, derived from proteolytic digestion, for subsequent MS analysis. Concurrently, the ability and selectivity of the synthesized receptors and functionalized NPs will be evaluated via spectroscopic analyses. The ENRICH network gathers the expertise required to tackle this challenge. The consortium is composed of 9 high-level academic research groups from 2 different continents (Europe and America) and 2 highly innovative companies. By the seconding of 87 ERs/ERSs across Europe and worldwide, the aim is to capitalize on the consortium expertise in complementary fields such as chemical synthesis, spectroscopy, and proteomics. The network promotes an effective integrate training of researchers, boosting their career development, and promotes collaborations between the partners. The direct involvement of industries guarantees the timely exploitation of the results from research laboratories to innovative products.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics DNA
- medical and health sciences clinical medicine endocrinology diabetes
- medical and health sciences clinical medicine psychiatry schizophrenia
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2022-SE-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
43121 PARMA
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.