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Novel Biophysical Tools to Measure Multiple Parameters In The Same Cell

Project description

Identification of cell mechanobiology biomarkers

Physical forces and mechanical properties influence the behaviour, function, and development of cells and organisms. Biophysical biomarkers, including the ability of a cell to deform under pressure, highlight vital changes in disease progression and can be used for early disease detection. Despite current microfluidic techniques allowing high-throughput measurements, they fall short in providing comprehensive, multi-feature data from the same cell, hindering insights in mixed cell populations. Funded by the Marie Skłodowska-Curie Actions programme, the SameMultiPhys project aims to create custom microfluidic systems capable of measuring multiple cellular features simultaneously. The work is expected to deepen the understanding of cell mechanobiology, advancing biomarker identification for improved diagnostics.

Objective

Biophysical biomarkers of cell state can reveal physiologically relevant changes that occur during disease progression. For example, cell deformability, cytoskeletal and nuclear organization, and macromolecular crowding are biophysical parameters implicated in migration and growth, which are essential processes for cellular functions. As biophysical parameters reflect physio-pathological cell states, they have the potential to be used as biomarkers for early diagnostics and clinical treatments in medicine. Current techniques based on microfluidics can be used to perform biophysical measurements in a high-throughput manner. However, these systems are not able to provide multiparameter, biophysical and same-cell measurements, making it difficult to find insightful relationships in heterogenous cellular mixtures.

SameMultiPhys will develop and produce customized microfluidic systems to measure multiple features in the same cells and evaluate their potential applications as biophysical biomarkers. These new technologies will allow us to study multiscale biological questions of immune T cells from an interdisciplinary perspective and other cell lines. This project aims to facilitate the transfer of knowledge and resources between institutions with expertise in microengineering, materials science, chemistry, mathematics, biology, and biophysics to promote collaboration between researchers, and to develop innovative microfluidic technologies for mechanobiology. The project will involve state-of-the-art microfabrication techniques, deep-learning analysis of images, and computational modelling to develop and validate the technologies developed. The ultimate goal of this project is to gain a deeper understanding of the mechanobiology of cells, enabling progress toward more effective identification of biomarkers.

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HORIZON-TMA-MSCA-SE - HORIZON TMA MSCA Staff Exchanges

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Call for proposal

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(opens in new window) HORIZON-MSCA-2022-SE-01

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Coordinator

UNIVERSIDAD POLITECNICA DE MADRID
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 69 000,00
Address
CALLE RAMIRO DE MAEZTU 7 EDIFICIO RECTORADO
28040 MADRID
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Higher or Secondary Education Establishments
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Participants (3)

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