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Robust industrial biocatalysts with peroxygenase, phenol-oxidase or furfuryl-oxidase activities from bacterial and fungal hosts

Objective

Industrial Biotechnology is key to maintain Europe's leading position in the global bio-based market and help the transition to a green and circular economy. Fostering investment in innovative technologies like those to be demonstrated in ROBUSTOO and supporting biotech SMEs will contribute to this endeavour. We aim to capitalise the results from previous EU projects that showed the applicability of three oxidative enzymes -unspecific peroxygenases (UPOs), laccases and hydroxymethylfurfural oxidases (HMFOs)- to provide novel and greener production of bio-based chemicals and materials. To exploit their full industrial potential, we will undertake the large-scale production of the recombinant enzymes and the development of new robust variants adapted to the demanding operating conditions. We will benefit from the most advanced technologies and know-how of the ROBUSTOO consortium to conduct computational enzyme bioprospecting and design (BSC), develop microbial strains for industrial enzyme production and engineer improved enzymes, followed by optimisation and pilot demonstration of target enzymatic transformations by biotech SMEs (Metgen, Gecco, bisy, and InnoSyn), and research/technological centres (CIB, UAB, IRNAS, and FCBA), concluding with environmental & techno-economical assessment and exploitation of the developed technologies (ITB). The new enzymes and bio-transformations will represent biotechnology breakthrough solutions for: i) conversion of industrial lignins modified by laccase into 100% bio-based resins as adhesives of wood panels and 3D printing additives, increasing the commercial value of existing lignin products; ii) production of intermediate and fine chemicals difficult to achieve by chemical synthesis through regio-/stereo-selective oxygenations of lipophilic substrates with UPOs; and iii) synthesis of 2,5-furandicarboxylic acid building block from 5-hydroxymethylfurfural using HMFOs, as a sustainable alternative to chemical catalytic processes.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-IA - HORIZON Innovation Actions

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-CL6-2023-CIRCBIO-01

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 470 000,00
Address
CALLE SERRANO 117
28006 MADRID
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 470 000,00

Participants (8)

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