A Complete Transformation PAth for C-C backboned plastic wastes to high-value Chemicals and materials

Project Information

ACTPAC

Grant agreement ID: 101135289

DOI

10.3030/101135289

EC signature date 17 November 2023

Start date 1 January 2024

End date 31 December 2027

Funded under

• Food, Bioeconomy Natural Resources, Agriculture and Environment

Total cost

No data

EU contribution

€ 4 765 580,92

Coordinated by

AARHUS UNIVERSITET
Denmark

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Deliverables

Documents, reports (5)

Production of 3 polyesters through an organometallic route at the gram scale

Bulk polymerization of the mixtures of diols and diacids (at the stoichiometry of the reactive functions) will be first implemented at high temperatures above the melting points of the monomer blends in the presence of efficient classical organometallic catalysts like ((Ti(OBu)4) to produce 3 polyesters at few g scale. The processes of polymerization will be also investigated by comparing conventional mechanical stirring to extrusion process with the objective to achieve full conversion.TASK 6.1

Results on characterization of CYP153 orthologs for their capacity of C6-C12 alkane to α, ω-diol and -diacid transformation

After activity and selectivity screening (chain length and regioselectivity) of 6-8 different CYP153 enzyme orthologs (enzymes from different organisms), we will choose the best 2-3 orthologs that can either alone or in cascade produce α, ω-diols and diacids from C6-C12 alkanes efficiently (i.e. high substrate loading, high TTN, high overall STY, high stability). The data on best orthologs will be instrumental for protein engineering efforts in Task 3.2.TASK 3.1

Methods established for the polycondensation of the developed long chain monomers

Polymer synthesis methodologies for the enzymatic (using lipase and cutinase enzymes) polycondensation of biobased building blocks (C6-C18 diacids/diols), which will be carried out in either a one-step or a two-step melt or solution polycondensation procedure, depending on the type of monomer and catalyst used. The choice of catalyst and exact procedure will depend on the co-monomer composition.TASK 8.1.

Successful genetic engineering of ATCC 20962 towards efficient diacid production

The genetic engineering tools for C. viswanathii (e.g. transformation, CRISPR-Cas) will be optimized for efficient genetic engineering. Targets for further optimisation such as CYP enzymes will be identified to realise an efficient conversion of specifically long chain alkanes.TASK 4.1

Successfully designed alkane converting bacterial strains for mid to long-chain diol production

Mainly the promising Gram-positive bacterial strain Paenibacillus polymyxa will be genetically engineered towards a mid to long-chain alkane conversion strain, but also other promsisng candidates will be evaluatet. Thus, the design of a novel bacterial strain to produce long-chain diols from alkanes will be completed.TASK 4.2

Websites, patent fillings, videos etc. (1)

Initiation of project’s electronic communication tools

Website and social media accounts for the project will become active with introductory materials.TASK 13.2

Data Management Plan (1)

Data management plan (initial)

A comprehensive initial data management plan for the handling/(re)use of data to be produced during the course of the project.TASK 13.4

Publications

Peer reviewed articles (1)

De Novo Synthesis of Perdeuterated Phosphoinositide by Installing a Non-native Phospholipid Biopathway in <i>E. coli</i>

Author(s): Aske Merrild, Niels Krabbe Johnsen, Mingliang Zhang, Oliver Bogojevic, Yi Ouyang, Zheng Guo
Published in: ACS Synthetic Biology, Issue 13, 2024, ISSN 2161-5063
Publisher: American Chemical Society (ACS)
DOI: 10.1021/ACSSYNBIO.4C00413