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Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .
Livrables
Bulk polymerization of the mixtures of diols and diacids (at the stoichiometry of the reactive functions) will be first implemented at high temperatures above the melting points of the monomer blends in the presence of efficient classical organometallic catalysts like ((Ti(OBu)4) to produce 3 polyesters at few g scale. The processes of polymerization will be also investigated by comparing conventional mechanical stirring to extrusion process with the objective to achieve full conversion.TASK 6.1
Results on characterization of CYP153 orthologs for their capacity of C6-C12 alkane to α, ω-diol and -diacid transformation (s’ouvre dans une nouvelle fenêtre)After activity and selectivity screening (chain length and regioselectivity) of 6-8 different CYP153 enzyme orthologs (enzymes from different organisms), we will choose the best 2-3 orthologs that can either alone or in cascade produce α, ω-diols and diacids from C6-C12 alkanes efficiently (i.e. high substrate loading, high TTN, high overall STY, high stability). The data on best orthologs will be instrumental for protein engineering efforts in Task 3.2.TASK 3.1
Methods established for the polycondensation of the developed long chain monomers (s’ouvre dans une nouvelle fenêtre)Polymer synthesis methodologies for the enzymatic (using lipase and cutinase enzymes) polycondensation of biobased building blocks (C6-C18 diacids/diols), which will be carried out in either a one-step or a two-step melt or solution polycondensation procedure, depending on the type of monomer and catalyst used. The choice of catalyst and exact procedure will depend on the co-monomer composition.TASK 8.1.
Successful genetic engineering of ATCC 20962 towards efficient diacid production (s’ouvre dans une nouvelle fenêtre)The genetic engineering tools for C. viswanathii (e.g. transformation, CRISPR-Cas) will be optimized for efficient genetic engineering. Targets for further optimisation such as CYP enzymes will be identified to realise an efficient conversion of specifically long chain alkanes.TASK 4.1
Successfully designed alkane converting bacterial strains for mid to long-chain diol production (s’ouvre dans une nouvelle fenêtre)Mainly the promising Gram-positive bacterial strain Paenibacillus polymyxa will be genetically engineered towards a mid to long-chain alkane conversion strain, but also other promsisng candidates will be evaluatet. Thus, the design of a novel bacterial strain to produce long-chain diols from alkanes will be completed.TASK 4.2
Website and social media accounts for the project will become active with introductory materials.TASK 13.2
A comprehensive initial data management plan for the handling/(re)use of data to be produced during the course of the project.TASK 13.4
Publications
Auteurs:
Aske Merrild, Niels Krabbe Johnsen, Mingliang Zhang, Oliver Bogojevic, Yi Ouyang, Zheng Guo
Publié dans:
ACS Synthetic Biology, Numéro 13, 2024, ISSN 2161-5063
Éditeur:
American Chemical Society (ACS)
DOI:
10.1021/ACSSYNBIO.4C00413
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