Key impacts of UNLOOC technology are: i) More efficient drug development, ii) Improved drug safety, iii) Reduced reliance on animal testing, iv) Personalised medicine, v) New insights into disease mechanisms, vi) New release procedures for drugs and chemicals, vii) New tools to release chemicals. Towards these goals all technical WPs and UCs have delivered significant impact: WP2 covered the roadmaps for all technologies involved, sensors as well as components and modules (D2.1 – D2.4) the requirements for all UCs (D2.4 – 2.10) the validation plan (D2.10) and requirements for the multi-level data analysis (D2.11) serving as foundation for all technical work and impact generated. WP3 developed a multitude of hardware from sensor, microfluidic devices, instrument modules, optical elements, electronics or biological and chemical components. Instrument modules and microfluidics together with subcomponents are summarized in D3.1. WP3 hardware development and its evaluation resulted already in three PEER reviewed publications. WP 4 focussing at software and AI compiled the roadmap guiding the development for this WP and its interactions (D4.8) as well as the data management definition report (D4.2). The system level development in WP5 just started and the main work will be carried out in year 2 and 3, same is valid for WP6. WP7 covers data security, data management and ethics and is interlinked with the work in the technical WPs. Main scientific impact that should be highlighted are the realization of a 4-donor-plate in UC1, lipidomics, RAMAN spectroscopy and lattice light sheet results on the analytical end in UC1. UC2a achieved the release of the first integrated SOMP-design (singel-organ multiwell plate) and provided minimized versions to the partners to start with the integration of epithelium- and cancer-cells. UC2b (MOMP – Multi-organ multi-well plate) can report a a scaled-down format for flexible integration and sensor testing that might serve as development platform for further projects. Special highlight is the development of a membrane not only having defined pore sized, but also a defined position and these membranes can be stacked together forming various multilayer pore-size arrays. UC3 (skin-on-chip) highlights the microfluidic modules for the various sensors in this UC ranging from electrochemical-, TEER to optical sensors and the development of special electronic features. Two of the three PEER reviewed papers resulted from UC3. UC4 (Blood-brain-barrier) provided the third scientific publication and has to report a variety of prototyped organ-on-chip modules, sensor developments, an incubator prototype as well as on application data and data analysis. UC5 (lung-on-chip) scientific impact includes a prototyped perfusion lid, the proof-of-concept for bio-impedance measurements, implementation of donor collection (two hospitals agreed to provide 40 donors), and finally UC5 provided data for the analytical engine and served as first test case giving guidance to the other UCs.
