Project description
Decoding plant resilience to heat stress
When plants confront heat stress, they experience a cascade of responses, including the simultaneous surge of reactive oxygen species (ROS) and the formation of stress granules (SGs). ROS, particularly hydrogen peroxide (H2O2), are known to trigger oxidative post-translational modifications (Oxi-PTMs) in proteins, while elevated temperatures induce the emergence of enigmatic SGs in the cytosol through liquid-liquid phase separation (LLPS). Traditionally seen as independent events, recent studies suggest a deeper connection between ROS accumulation and SG formation, especially regarding Oxi-PTMs’ role in regulating protein LLPS. With the support of the Marie Skłodowska-Curie Actions programme, the REPHASE project sets out to unravel this intricate relationship by probing the redox biology of SGs during heat stress. It will use cutting-edge biosensors and proteomic probes.
Objective
The proposed project explores the intriguing possibility of reactive oxygen species (ROS) regulating the formation of stress granules (SGs) during heat stress. Heat stress triggers the rapid accumulation of ROS, with H2O2 playing a pivotal role as a signalling molecule that induces oxidative post-translational modifications (Oxi-PTMs) to regulate protein activities. On the other hand, an increase in temperature leads to the formation of enigmatic droplets in the cytosol known as SGs. These membraneless organelles (MLOs) arise through liquid-liquid phase separation (LLPS), enabling the concentration of proteins, mRNA, and metabolites to facilitate cellular adaptation. ROS accumulation and SGs formation have traditionally been viewed as independent events in the heat stress response. However, recent studies have shed light on the significance of Oxi-PTMs as regulators of protein LLPS in plants.
Moreover, my preliminary experiments have demonstrated that the chemical blocking of Oxi-PTMs impairs SG formation during heat stress. I propose that ROS accumulation during heat stress induces Oxi-PTMs on proteins, promoting LLPS and facilitating SG formation. The REPHASE project aims to address this hypothesis through two main objectives. Firstly, I will investigate the dynamics of the redox state within SGs during heat stress. Secondly, I will map the landscape of Oxi-PTMs in SGs shedding light on their distribution and functional implications. To achieve these goals, the fluorescent redox biosensor roGFP2-Orp1 and the proteomic OxiTRAP probe YAP1 will be targeted to SGs. Utilizing these innovative methods will enable a comprehensive exploration of the redox biology of SGs, leveraging the state-of-the-art proteomic core and live cell imaging facilities available at the host institute. By bridging the gap between redox signalling and SGs, the REPHASE project promises to provide valuable insights into the mechanisms underlying plant adaptation to environmental stress.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors biosensors
- natural sciences biological sciences biochemistry biomolecules proteins
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
9052 ZWIJNAARDE - GENT
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.