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Deciphering the molecular mechanism of Eleos, a conserved immune system across the tree of life

Project description

Insight into the evolution of antiviral immunity

Both eukaryotes and prokaryotes face the threat of viral infection and have therefore evolved various defence mechanisms. Accumulating evidence suggests that certain components of the innate immune system in higher eukaryotes have evolved from bacterial components. Funded by the Marie Skłodowska-Curie Actions programme, the ECoToL project focuses on Eleos, a newly identified anti-viral system in prokaryotes, which shares similarities with eukaryotic GTPase immunity-associated proteins. By utilising large genomic datasets and a suite of experimental techniques, researchers aim to shed light into the evolution and function of Eleos and enhance our understanding of immune defence systems. Project findings can pave the way for novel treatments against viral infections and for enhancing global health.

Objective

Sensing viral infections and mounting a response is crucial for all domains of life. Many immune mechanisms in higher eukaryotes were thought to be evolutionary innovations, but recent research reveals that some components of the innate immune system have evolved from bacterial genes. Eleos, a novel anti-viral system in prokaryotes, shares similarities with eukaryotic GIMAPs (GTPase immunity-associated proteins), yet their evolutionary history and anti-viral mechanisms remain unknown.
ECoToL's objectives are threefold:
1. Identify Eleos systems, study their architecture, and determine their distribution in bacteria.
2. Explore the molecular mechanism by which bacterial Eleos confers resistance against phages.
3. Investigate when and how Eleos was acquired in eukaryotes, assess its distribution, and examine the conservation of the mechanism in eukaryotes.
We will analyze large genome datasets to trace Eleos system evolution across different life domains. Additionally, we will identify phages Eleos defends against and the mechanisms by which phages evade this defense. Biochemical, biophysical, and structural biology methods will illuminate the molecular mechanisms in bacterial Eleos. Further genomic analysis and experimental methods will answer whether this mechanism is conserved in higher eukaryotes.
ECoToL is a multidisciplinary project pioneering comparative immunology between prokaryotes and eukaryotes. This innovative concept aims to enhance our understanding of immune systems, which is critical for staying ahead of viral threats, improving viral infection treatments, reducing viral resistance, and enhancing global health security.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

INSTITUT PASTEUR
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 211 754,88
Address
RUE DU DOCTEUR ROUX 25-28
75724 Paris
France

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Ile-de-France Ile-de-France Hauts-de-Seine
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