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Using Single-Cell Transcriptomics to identify the Diatom Silica Morphogenesis Machinery

Objective

Diatoms are a large group of unicellular, eukaryotic microalgae that biosynthesize SiO2 (silica)-based cell walls (frustules). They are ubiquitously present in aquatic habitats and key primary producers in the oceans, contributing 20% of the annual photosynthetic CO2 fixation on earth. The architectures of frustules are species specific, displaying intricate patterns of nano- and microscale features, and are believed to be of prime importance for the ecological success of diatoms. Furthermore, in biomineralization research, diatoms serve as model systems to reveal the mechanisms by which organisms are able to generate inorganic materials with complex architectures. Such insight holds the promise of gaining advanced capabilities to synthesize minerals with tailored properties using an environmentally benign processes. During the past two decades, numerous candidate proteins potentially involved in diatom silica biogenesis have been put forward, but only a small number of these have been confirmed to be required for this process. The DiaMorphy project aims to identify the complete set of proteins required for silica morphogenesis in the model diatom Thalassiosira pseudonana. To achieve this, the project will inverse the conventional approach by analyzing silica biogenesis in induced morphological mutants of T. pseudonana rather than in the wild-type. For the first time, single-cell transcriptomics will be applied to study gene expression at specific stages during silica biogenesis. Comparative analysis of the resulting, highly refined transcriptomics datasets, is expected to 'crystallize' a comprehensive core set of silica morphogenesis genes. In proof-of-principle experiments, selected proteins from this core set, will then be functionally characterized in vivo using state-of-the art genetic engineering techniques.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

TECHNISCHE UNIVERSITAET DRESDEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 173 847,36
Address
HELMHOLTZSTRASSE 10
01069 DRESDEN
Germany

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Region
Sachsen Dresden Dresden, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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