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Exploring a click-to-release approach to uncage FAP-theranostics, based on TCO-radiotracers

Objective

Radioligand therapy has recently received renewed attention as promising treatment option to improve cancer patient outcome. Compared to conventional cancer treatments, this strategy involves a more specific targeting of therapeutic radionuclides to the tumor, which aims to increase treatment efficacy. Despite recent progress, currently available treatments show insufficient tumor killing and normal tissue toxicity, and we hypothesize that a pretargeted click-to-release (C2R) strategy can bridge this gap. Within this strategy, we propose to explore Fibroblast Activation Protein (FAP) inhibitors as tumor targeting vectors, to deliver a therapeutic radionuclide fragment intracellularly using cell permeable, cleavable transcyclooctene (dcTCO) constructs and compare it with conventional dTCOs. To reinsure intracelular release, after C2R, we designed new structures containing an RGD peptide (with integrin v3 binding specificity). Our molecular constructs also aim to prevent FAP efflux, by using a guanidinium and nicotinoyl residualizing groups.
This project will evaluate the reactivity, in vitro stability, logD and radiochemical yield of the new molecules, and the C2R efficiency of the dcTCOs. A FAP inhibitor (dimer) will be weaponized with a tetrazine handle to be the targeting vector and biorthogonal pair of these new constructs, and it's affinity and selectivity will be evaluated in vitro, against other proteases. The dcTCOs and dTCOs with better reaction kinetics and C2R efficiency (dcTCOs) will be selected for the development of 18F- and 125I-radiolabeled probes. The celular uptake and internalization will be evaluated on tumor cells (FAP positive cells, integrin v3 positive cells, both FAP and v3 positive).
This project is a innovative strategy to improve the radiation dose delivered to the tumor and improving treatment outcome, while reducing radiotoxicity and achieving better treatment tolerability.

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Topic(s)

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITEIT ANTWERPEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 175 920,00
Address
PRINSSTRAAT 13
2000 Antwerpen
Belgium

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Region
Vlaams Gewest Prov. Antwerpen Arr. Antwerpen
Activity type
Higher or Secondary Education Establishments
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Total cost

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